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Browsing Electronic Theses and Dissertations by Duke Department

Browsing Electronic Theses and Dissertations by Duke Department "Biochemistry"

Now showing items 41-52 of 52

Structural and Kinetic Characterization of RNA Polymerase II C-Terminal Domain Phosphatase Ssu72 and Development of New Methods for NMR Studies of Large Proteins

Werner-Allen, Jonathan (2011)

Ssu72 is a protein phosphatase that selectively targets phosphorylated serine residues at the 5th position (pS5) in the heptad repeats of the C-terminal domain (CTD) of RNA polymerase II, in order to regulate the ...
Structural Biochemistry and Inhibition of CaaX Protein Prenyltransferases From Human Pathogens

Hast, Michael Alan (2010)

Protein prenylation is a post-translational lipid modification required for proper function by over 100 proteins in the eukaryotic cell. Proteins that receive this modification mediate a wide variety of functions in ...
Structural Mechanisms of Accurate and Mutagenic DNA Replication

Wang, Weina (2012)

High-fidelity DNA polymerases achieve remarkable accuracy by exhibiting exquisite nucleotide substrate selection mechanisms. DNA polymerases not only discriminate highly against base-pair mismatches, but also show a ...
Structural Studies of Arabidopsis Thaliana Inositol Polyphosphate Multi-Kinase

Endo-Streeter, Stuart Tamotsu (2009)

Inositol Polyphosphate Multi-Kinase (IPMK, also known as ArgRIII, Arg82, and IPK2) is a central component of the inositol signaling system, catalyzing the phosphorylation of at least four different inositol polyphosphate ...
Structure-Function Studies in Sulfite Oxidase with Altered Active Sites

Qiu, James (2009)

Sulfite oxidase, a metabolically important enzyme, catalyzes the physiologically critical conversion of sulfite to sulfate in the terminal step of the degradation of sulfur containing compounds. The enzyme has been the ...
Structure-Guided Development of Novel LpxC Inhibitors

LEE, CHUL-JIN (2013)

The incessant increase of antibiotic resistance among Gram-negative pathogens is a serious threat to public health worldwide. A lack of new antimicrobial agents, particularly those against multidrug-resistant Gram-negative ...
Studies on adenine nucleotide states and their role in human MutSα function

Asllani, Lored (2012)

Mismatch repair is a conserved pathway that corrects errors resulting from the misincorporation of bases during DNA synthesis or from the production of damaged bases. The mismatch repair pathway is initiated by MutS ...
Studies on Redesign and Solution Structure Determination of Nonribosomal Peptide Synthetases and Redox Regulation of Phosphatases

Chen, Cheng-Yu (2013)

We present a computational structure-based redesign of the phenylalanine adenylation domain of the non-ribosomal peptide synthetase (NRPS) enzyme gramicidin S synthetase A (GrsA-PheA) for a set of non-cognate substrates ...
Topoisomerase III-alpha in Double Holliday Junction Dissolution

Chen, Stefanie Lynn Hartman (2012)

Topoisomerase IIIα (Top3α) is an essential component of the double Holliday junction (dHJ) dissolvasome complex in metazoans. Previous work has shown that Top3α and Bloom's helicase (Blm) are able to ...
Ubiquitin Recognition by Ubiquitin-Binding Domains in Y-Family DNA Polymerases

Bomar, Martha Grier (2009)

Translesion synthesis (TLS) is a specialized type of DNA repair for bypassing DNA damage at the stalled replication fork. Because the TLS polymerases (mainly from the Y-family of polymerases) are characterized by more ...
Using Protein-Likeness to Validate Conformational Alternatives

Keedy, Daniel Austin (2012)

Proteins are among the most complex entities known to science. Composed of just 20 fundamental building blocks arranged in simple linear strings, they nonetheless fold into a dizzying array of architectures that carry ...
ZFP36L3: a Unique Member of the Tristetraprolin Family of RNA-Binding Tandem Zinc Finger Proteins

Frederick, Elizabeth (2009)

Members of the tristetraprolin (TTP) family of CCCH tandem zinc finger proteins bind to AU-rich elements in the 3' untranslated regions of certain cellular mRNAs, leading to their deadenylation and destabilization. ...