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dc.contributor.advisor Linden, Karl G en_US
dc.contributor.author Eischeid, Anne en_US
dc.date.accessioned 2009-05-01T18:42:50Z
dc.date.available 2011-07-26T04:30:03Z
dc.date.issued 2009 en_US
dc.identifier.uri http://hdl.handle.net/10161/1256
dc.description Dissertation en_US
dc.description.abstract <p>The adenoviruses are nonenveloped double stranded DNA viruses, which cause enteric dysentary and respiratory infection. Adenovirus has become a focus of the water treatment community because of its apparent resistance to ultraviolet disinfection; it is the basis for stringent new EPA regulations regarding all viruses in both surface and ground waters. Most of the work done so far, however, has involved the use of monochromatic (254 nm) low pressure (LP) UV sources and subsequent assay of viral infectivity in cell culture models. LP UV lamps primarily damage DNA, while polychromatic UV sources may damage other parts of the virus as well. Recent research has shown that these newer, polychromatic UV sources--such as medium pressure (MP) UV--are more effective than monochromatic LP UV for disinfection of adenovirus. The objectives of this work were to study adenoviral response to UV using both LP and MP UV as well as using both standard cell culture infectivity assays and more direct methods of assessment based on molecular biology. These include quantitative long PCR for assessment of DNA damage and SDS-PAGE for assessment of protein damage; transmission electron microscopy was used to examine the structure of UV treated viral particles. This work was only the second significant study to show the response of adenoviruses to medium pressure UV and the first to thoroughly examine the response of adenoviruses to both LP and MP UV using cell culture-independent methods. Results confirm that adenovirus is sensitive to MP UV when assayed in cell culture; they show that LP and MP UV are equally effective at inducing damage to the adenoviral genome and that MP UV is more effective than LP UV at damaging the viral proteins. This work helps deepen our understanding of UV disinfection of adenovirus.</p> en_US
dc.format.extent 1035930 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Engineering, Environmental en_US
dc.subject Biology, Microbiology en_US
dc.subject adenovirus en_US
dc.subject DNA en_US
dc.subject infectivity en_US
dc.subject PCR en_US
dc.subject protein en_US
dc.subject UV en_US
dc.title Fundamental Mechanisms in the Extreme UV Resistance of Adenovirus en_US
dc.type Dissertation en_US
dc.department Civil and Environmental Engineering en_US
duke.embargo.months 24 en_US

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