Abstract:
New mathematical models are presented for analysis of fluorescence recovery
after photobleaching (FRAP) data to account for the conical geometry of photobleaching,
for spatial variations in binding of fluorescent molecules, and for directed transport of
fluorescent molecules. A fast computational method for calculation of fluorescence
recovery with this conical geometry is presented, and is shown to be applicable in
calculations with spatial variations. Some of the new models are applied to experimental
data from a previously published paper (Biophys. J. 95:3048) and are found to reinforce
the existing conclusions, and furthermore to suggest that the protein γ-tubulin is likely to
be uncomplexed in the cytoplasm.
