Improving the Accuracy and Scope of Quantitative FRAP Analysis
Date
2009-05-04
Authors
Journal Title
Journal ISSN
Volume Title
Repository Usage Stats
views
downloads
Abstract
New mathematical models are presented for analysis of fluorescence recovery after photobleaching (FRAP) data to account for the conical geometry of photobleaching, for spatial variations in binding of fluorescent molecules, and for directed transport of fluorescent molecules. A fast computational method for calculation of fluorescence recovery with this conical geometry is presented, and is shown to be applicable in calculations with spatial variations. Some of the new models are applied to experimental data from a previously published paper (Biophys. J. 95:3048) and are found to reinforce the existing conclusions, and furthermore to suggest that the protein γ-tubulin is likely to be uncomplexed in the cytoplasm.
Type
Department
Description
Provenance
Citation
Permalink
Citation
Hallen, Mark (2009). Improving the Accuracy and Scope of Quantitative FRAP Analysis. Honors thesis, Duke University. Retrieved from https://hdl.handle.net/10161/1277.
Dukes student scholarship is made available to the public using a Creative Commons Attribution / Non-commercial / No derivative (CC-BY-NC-ND) license.