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dc.contributor.advisor Klingensmith, John HASH(0x1f3d0a4) en_US
dc.contributor.author Custer, Laura Mary en_US
dc.date.accessioned 2009-12-18T16:26:18Z
dc.date.available 2011-12-31T05:30:07Z
dc.date.issued 2009 en_US
dc.identifier.uri http://hdl.handle.net/10161/1630
dc.description Dissertation en_US
dc.description.abstract <p>The actin cytoskeleton is a fundamental component of the cell and is involved in many processes, including cell division, cell migration, vesicle trafficking and cell polarity. The actin cytoskeleton has a very important role in embryogenesis as the cells within developing tissues proliferate, migrate, interpret extracellular cues, and shape complex tissues. The molecules that help the cell to interpret their environment and turn those cues into morphological changes are of great interest. One protein which may be involved in this manner is Cordon-bleu (Cobl). </p><p>In mouse embryos, <italic>Cobl's</italic> expression pattern resembles that of important developmental genes, is restricted to distinct domains, and changes dynamically throughout development as tissues are formed. While it is known that <italic>Cobl</italic> expression is regulated by developmental signaling pathways such as Shh and BMP, its molecular function at the cellular level remains elusive. In this study, we have identified molecular functions of Cobl. Cobl has C-terminal Wasp Homology-2 (WH2) domains which bind actin and nucleate new actin filaments in <italic>in vitro</italic> polymerization assays. Using cultured cells, we have determined that Cobl is involved in cytoskeletal remodeling during neurite branching and epithelial cell migration. We also demonstrate that Cobl interacts with the Syndapin family of adaptor proteins that link endocytosis and vesicle trafficking. Cobl colocalizes with Sdp2 in cultured epithelial cells and similarly localizes with Sdp1 and Sdp2 in developing mouse embryos. The localization of Cobl or Sdp2 in cultured epithelial cells is dependent on the other, as demonstrated using shRNA knockdown. </p><p>Previous studies demonstrated that a hypomorphic allele of <italic>Cobl</italic> interacts genetically with <italic>Looptail</italic>, in midbrain neurulation. <italic>Looptail</italic> mutants are deficient in the gene <italic>Vangl2</italic>, a member of the planar cell polarity pathway that coordinates the morphogenesis of a sheet of cells. To discover other roles for <italic>Cobl</italic> in the developing mouse, we have generated a conditionally null allele of <italic>Cobl</italic>. We find that outbred <italic>Cobl</italic> homozygous mutants are viable, but that they have inner ear defects. Together, our studies demonstrate that Cobl is a tissue-specific actin nucleator whose localization is regulated by its interaction with Syndapins and which functions in the development of sensory epithelia.</p> en_US
dc.format.extent 90775589 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Biology, Cell en_US
dc.subject actin en_US
dc.subject cilia en_US
dc.subject Cordon en_US
dc.subject bleu en_US
dc.subject mouse en_US
dc.subject Syndapin en_US
dc.title Functional Analysis of the Cordon-bleu Protein in Mouse en_US
dc.type Dissertation en_US
dc.department Cell Biology en_US
duke.embargo.months 24 en_US

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