Show simple item record

dc.contributor.advisor Dietrich, Fred S.
dc.contributor.advisor Heitman, Joseph
dc.contributor.advisor Lutzoni, Francois
dc.contributor.advisor McCusker, John
dc.contributor.advisor Mitchell, Thomas
dc.contributor.advisor Rausher, Mark
dc.contributor.author Hall, Charles Robert
dc.date 2007
dc.date.accessioned 2007-05-10T14:55:20Z
dc.date.available 2007-05-10T14:55:20Z
dc.date.issued 2007-05-10T14:55:20Z
dc.identifier.uri http://hdl.handle.net/10161/202
dc.description Dissertation
dc.description.abstract The genomes of the hemiascomycetes Saccharomyces cerevisiae and Ashbya gossypii have been completely sequenced, allowing a comparative analysis of these two genomes, which reveals that a small number of genes appear to have entered these genomes as a result of horizontal gene transfer from bacterial sources. One potential case of horizontal gene transfer in A. gossypii and 10 potential cases in S. cerevisiae were identified, of which two were investigated further. One gene, encoding the enzyme dihydroorotate dehydrogenase (DHOD), is potentially a case of horizontal gene transfer, as shown by sequencing of this gene from additional bacterial and fungal species to generate sufficient data to construct a well-supported phylogeny. The DHOD-encoding gene found in S. cerevisiae, URA1 (YKL216W), appears to have entered the Saccharomycetaceae after the divergence of the S. cerevisiae lineage from the Candida albicans lineage and possibly since the divergence from the A. gossypii lineage. This gene appears to have come from the Lactobacillales, and following its acquisition the endogenous eukaryotic DHOD gene was lost. It was also shown that the bacterially derived horizontally transferred DHOD is required for anaerobic synthesis of uracil in S. cerevisiae. The other gene discussed in detail is BDS1, an aryl- and alkyl-sulfatase gene of bacterial origin that we have shown allows utilization of sulfate from several organic sources. Among the eukaryotes, this gene is found in S. cerevisiae and Saccharomyces bayanus and appears to derive from the alpha-proteobacteria. en
dc.format.extent 15275878 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.subject horizontal gene transfer en
dc.subject lateral gene transfer en
dc.subject Saccharomyces cerevisiae en
dc.subject biotin en
dc.subject uracil en
dc.subject sulfatase en
dc.title The Contribution of Horizontal Gene Transfer to the Evolution of Fungi. en
dc.type Dissertation en
dc.department Molecular Genetics and Microbiology

Files in this item

This item appears in the following Collection(s)

Show simple item record