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dc.contributor.advisor Amrein, Hubert O en_US
dc.contributor.author Hendricks, Dianne Grayce en_US
dc.date.accessioned 2010-05-10T19:55:33Z
dc.date.available 2010-05-10T19:55:33Z
dc.date.issued 2009 en_US
dc.identifier.uri http://hdl.handle.net/10161/2362
dc.description Dissertation en_US
dc.description.abstract <p>In Drosophila melanogaster, where males are XY and females are XX, dosage compensation is acheived by approximately two-fold upregulation of transcription of the single male X chromosome. This upregulation is mediated by the dosage compensation complex (DCC), which is assembled in a sequential manner on the male X chromosome and is composed of the two noncoding roX (RNA on the X) RNAs and at least five proteins, including the RNA helicase Maleless (MLE). MLE contains two highly conserved double stranded RNA binding domains (DRBDs) at the N terminus. We investigated the roles of the roX RNAs and MLE helicase through experiments using classical genetic methods to generate and analyze the effects of mutants and mutant transgenes, immunolocalization experiments to study MSL protein and roX RNA to chromosomes. For the first time in vivo, we demonstrate that MLE associates with double stranded RNA in a sequence non-specific manner that is independent of other DCC components. Importantly, we find that the DSRBDs of MLE are essential for dosage compensation but are not required for MLE localization to the male X chromosome. We propose that although the DSRBDs are not essential for ds RNA binding, they may act synergistically with other domains of MLE or other MSLs to associate with RNA in vivo. We propose that a MLE/ roX RNA association involving secondary structure formed by the roX RNAs may be involved in the assembly, stabilization, or function of the DCC.</p> en_US
dc.format.extent 1502842 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Biology, Genetics en_US
dc.subject dosage compensation en_US
dc.subject double stranded RNA binding domain en_US
dc.subject Drosophila en_US
dc.subject maleless en_US
dc.subject MLE en_US
dc.subject roX en_US
dc.title Investigation of the roX RNAs and the RNA Helicase MLE in Dosage Compensation in Drosophila melanogaster en_US
dc.type Dissertation en_US
dc.department Molecular Genetics and Microbiology en_US
duke.embargo.months 12 en_US
dc.date.accessible 2010-05-18T05:00:39Z

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