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dc.contributor.advisor Rittschof, Dan en_US
dc.contributor.author Osterberg, Joshua Samuel en_US
dc.date.accessioned 2010-05-10T20:17:39Z
dc.date.available 2010-05-10T20:17:39Z
dc.date.issued 2010 en_US
dc.identifier.uri http://hdl.handle.net/10161/2460
dc.description Dissertation en_US
dc.description.abstract <p>Reactive oxygen species (ROS) are produced endogenously in all aerobes and are induced by environmental stressors. ROS oxidize and disable essential cellular components such as DNA, proteins, and lipid membranes. Exposure to metals, polycyclic aromatic hydrocarbons (PAHs), and some pesticides can induce oxidative stress in marine invertebrates. All aerobic organisms have a network of antioxidants and enzymes to quench ROS and prevent oxidative damage. This dissertation examines antioxidant and oxidative stress biomarkers in endemic molluscs and crabs from two natural extreme environments: deep-sea hydrothermal vents in the Lau and North Fiji Basin, and cold seeps in the Gulf of Mexico. In addition, the acute toxicity and sub-lethal effects of four insecticides and an herbicide are examined in the estuarine blue crab, <italics>Callinectes sapidus</italics>. Blue crabs are North Carolina's most important fishery species and are frequently found in agricultural drainage ditches, an example of an anthropogenic extreme environment. </p> <p>Total glutathione, catalase, superoxide dismutase, and lipid peroxidation levels were of the same respective order of magnitude in the two vent gastropods, <italics>Alviniconcha</italics> sp. and <italics>Ifremeria nautilei</italics>, and vent mussel, <italics>Bathymodiolus brevior</italics>. These biomarkers activities were similar to those from previous reports on Mid-Atlantic Ridge mussels, except for ~100-fold higher lipid peroxidation levels among Lau molluscs. Principal component analysis (PCA) of mollusc tissue-specific biomarker levels grouped individuals by species rather than by site. </p> <p>Biomarker levels in the seep mussels <italics>Bathymodiolus childressi, B. brooksi</italics>, and <italics>B. heckerae</italics> were similar across species except for elevated foot and gill cytosolic SOD in mussels from MC-640 compared to those from AC-645. PCA of seep mussel biomarker levels differentiated by species with <italics>B. childressi</italics> isolated from <italics>B. brooksi</italics> and <italics>B. heckerae</italics>. The addition of <italics>B. brevior</italics> biomarker data to the PCA showed them grouping around <italics>B. brooksi</italics> and <italics>B. heckerae</italics>. <italics>Bathymodiolus childressi</italics> is ancestral to the other species and contains only methanotrophic endosymbionts. Whether symbionts play a role in alleviating possible toxic conditions remains unknown.</p> <p>Pesticides were acutely toxic to blue crabs in the order of Lambda-cyhalothrin > imidacloprid &#8776; aldicarb > acephate &#8776; Roundup® (glyphosate). Megalopae were almost always more sensitive to pesticides than early stage juveniles. Commercial formations of pesticides generally showed similar toxicity to active ingredients alone. Exposure to LC<sub>20</sub> levels of acephate, aldicarb, imidacloprid and Roundup significantly increased the frequency of juvenile mortality after molting. There was no significant change in total glutathione or lipid peroxidation of exposed megalopae. Lambda-cyhalothrin-, imidacloprid-, and aldicarb-based products have the potential to cause acute toxicity and molting-related mortality in shallow creeks and ditches.</p> en_US
dc.format.extent 1026129 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Biology, Oceanography en_US
dc.subject Biology, Ecology en_US
dc.subject Biology, Physiology en_US
dc.subject Anitoxidant en_US
dc.subject Bathymodiolus en_US
dc.subject Callinectes sapidus en_US
dc.subject Hydrothermal vent en_US
dc.subject Oxidative stress en_US
dc.subject Toxicity en_US
dc.title Ecotoxicology of Natural and Anthropogenic Extreme Environments en_US
dc.type Dissertation en_US
dc.department Ecology en_US
duke.embargo.months 6 en_US
dc.date.accessible 2010-05-18T05:00:17Z

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