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dc.contributor.advisor Garcia-Blanco, Mariano en_US
dc.contributor.advisor Dewhirst, Mark en_US
dc.contributor.author Robinson, Timothy J. en_US
dc.date.accessioned 2010-05-10T20:17:44Z
dc.date.available 2012-05-01T04:30:05Z
dc.date.issued 2010 en_US
dc.identifier.uri http://hdl.handle.net/10161/2462
dc.description Dissertation en_US
dc.description.abstract <p>Alternative mRNA processing can result in the generation of multiple, qualitatively different RNA transcripts from the same gene and is a powerful engine of complexity in higher organisms. Recent deep sequencing studies have indicated that essentially all human genes containing more than a single exon generate multiple RNA transcripts. Functional roles of alternative processing have been established in virtually all areas of biological regulation, particularly in development and cancer. Changes in alternative mRNA processing can now be detected from over a billion dollars' worth of conventional gene expression microarray data archived over the past 20 years using a program we created called SplicerAV. Application of SplicerAV to publicly available microarray data has granted new insights into previously existing studies of oncogene over-expression and clinical cancer prognosis.</p> <p>Adaptation of SplicerAV to the new Affymetrix Human Exon arrays has resulted in the creation of SplicerEX, the first program that can automatically categorize microarray detected changes in alternative processing into biologically pertinent categories. We use SplicerEX's automatic event categorization to identify changes in global mRNA processing during B cell transformation and show that the conventional U133 platform is able to detect 3' located changes in mRNA processing five times more frequently than the Human Exon array.</p> en_US
dc.format.extent 6734277 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Biology, Bioinformatics en_US
dc.subject Affymetrix en_US
dc.subject Alternative Splicing en_US
dc.subject gene expression en_US
dc.subject microarray en_US
dc.subject SplicerAV en_US
dc.subject SplicerEX en_US
dc.title Detecting Changes in Alternative mRNA Processing From Microarray Expression Data en_US
dc.type Dissertation en_US
dc.department Molecular Cancer Biology en_US
duke.embargo.months 24 en_US

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