Browsing Duke Faculty Scholarship by Subject "h/d exchange"

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Mass Spectrometry-Based Thermal Shift Assay for Protein-Ligand Binding Analysis

West, Graham M.; DeArmond, Patrick D.; Fitzgerald, Michael (AMER CHEMICAL SOC, 2010)

Described here is a mass spectrometry-based screening assay for the detection of protein ligand binding interactions in multicomponent protein mixtures. The assay utilizes an oxidation labeling protocol that involves using ...
Thermodynamic Analysis of a Molecular Chaperone Binding to Unfolded Protein Substrates

Xu, Ying; Tang, Liangjie; Fitzgerald, Michael (AMER CHEMICAL SOC, 2010)

Molecular chaperones are a highly diverse group of proteins that recognize and bind unfolded proteins to facilitate protein folding and prevent nonspecific protein aggregation. The mechanisms by which chaperones bind their ...