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dc.contributor.author Todd, LR
dc.contributor.author Damin, MN
dc.contributor.author Gomathinayagam, R
dc.contributor.author Horn, SR
dc.contributor.author Means, AR
dc.contributor.author Sankar, U
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:42Z
dc.date.issued 2010-04-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20147447
dc.identifier E09-11-0937
dc.identifier.citation Mol Biol Cell, 2010, 21 (7), pp. 1225 - 1236
dc.identifier.uri http://hdl.handle.net/10161/3245
dc.description.abstract The relationship of mitochondrial dynamics and function to pluripotency are rather poorly understood aspects of stem cell biology. Here we show that growth factor erv1-like (Gfer) is involved in preserving mouse embryonic stem cell (ESC) mitochondrial morphology and function. Knockdown (KD) of Gfer in ESCs leads to decreased pluripotency marker expression, embryoid body (EB) formation, cell survival, and loss of mitochondrial function. Mitochondria in Gfer-KD ESCs undergo excessive fragmentation and mitophagy, whereas those in ESCs overexpressing Gfer appear elongated. Levels of the mitochondrial fission GTPase dynamin-related protein 1 (Drp1) are highly elevated in Gfer-KD ESCs and decreased in Gfer-overexpressing cells. Treatment with a specific inhibitor of Drp1 rescues mitochondrial function and apoptosis, whereas expression of Drp1-dominant negative resulted in the restoration of pluripotency marker expression in Gfer-KD ESCs. Altogether, our data reveal a novel prosurvival role for Gfer in maintaining mitochondrial fission-fusion dynamics in pluripotent ESCs.
dc.format.extent 1225 - 1236
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Mol Biol Cell
dc.relation.isversionof 10.1091/mbc.E09-11-0937
dc.subject Animals
dc.subject Cell Line
dc.subject Cytochrome Reductases
dc.subject Dynamins
dc.subject Embryonic Stem Cells
dc.subject GTP Phosphohydrolases
dc.subject Gene Expression Regulation, Developmental
dc.subject Genes, Dominant
dc.subject Golgi Apparatus
dc.subject Humans
dc.subject Membrane Potentials
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject Microscopy, Fluorescence
dc.subject Microtubule-Associated Proteins
dc.subject Mitochondria
dc.subject Models, Biological
dc.subject Oxidoreductases Acting on Sulfur Group Donors
dc.title Growth factor erv1-like modulates Drp1 to preserve mitochondrial dynamics and function in mouse embryonic stem cells.
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-4-1 en_US
duke.description.endpage 1236 en_US
duke.description.issue 7 en_US
duke.description.startpage 1225 en_US
duke.description.volume 21 en_US
dc.relation.journal Molecular biology of the cell en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20147447
pubs.issue 7
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pathology
pubs.publication-status Published
pubs.volume 21
dc.identifier.eissn 1939-4586

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