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dc.contributor.author Geonnotti, AR
dc.contributor.author Bilska, M
dc.contributor.author Yuan, X
dc.contributor.author Ochsenbauer, C
dc.contributor.author Edmonds, TG
dc.contributor.author Kappes, JC
dc.contributor.author Liao, HX
dc.contributor.author Haynes, BF
dc.contributor.author Montefiori, DC
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:19Z
dc.date.issued 2010-03
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20218881
dc.identifier.citation AIDS Res Hum Retroviruses, 2010, 26 (3), pp. 279 - 291
dc.identifier.uri http://hdl.handle.net/10161/3301
dc.description.abstract Bacterial lipopolysaccharide (endotoxin) is a frequent contaminant of biological specimens and is also known to be a potent inducer of beta-chemokines and other soluble factors that inhibit HIV-1 infection in vitro. Though lipopolysaccharide (LPS) has been shown to stimulate the production of soluble HIV-1 inhibitors in cultures of monocyte-derived macrophages, the ability of LPS to induce similar inhibitors in other cell types is poorly characterized. Here we show that LPS exhibits potent anti-HIV activity in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs) but has no detectable anti-HIV-1 activity in TZM-bl cells. The anti-HIV-1 activity of LPS in PBMCs was strongly associated with the production of beta-chemokines from CD14-positive monocytes. Culture supernatants from LPS-stimulated PBMCs exhibited potent anti-HIV-1 activity when added to TZM-bl cells but, in this case, the antiviral activity appeared to be related to IFN-gamma rather than to beta-chemokines. These observations indicate that LPS stimulates PBMCs to produce a complex array of soluble HIV-1 inhibitors, including beta-chemokines and IFN-gamma, that differentially inhibit HIV-1 depending on the target cell type. The results also highlight the need to use endotoxin-free specimens to avoid artifacts when assessing HIV-1-specific neutralizing antibodies in PBMC-based assays.
dc.format.extent 279 - 291
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof AIDS Res Hum Retroviruses
dc.relation.isversionof 10.1089/aid.2009.0186
dc.subject Antibodies, Monoclonal
dc.subject Antibodies, Neutralizing
dc.subject Cell Line, Tumor
dc.subject Chemokines, CC
dc.subject Equipment Contamination
dc.subject Escherichia coli
dc.subject HIV Infections
dc.subject HIV-1
dc.subject Humans
dc.subject Interferon-gamma
dc.subject Leukocytes, Mononuclear
dc.subject Lipopolysaccharides
dc.subject Neutralization Tests
dc.subject Salmonella enterica
dc.title Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production.
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-3-0 en_US
duke.description.endpage 291 en_US
duke.description.issue 3 en_US
duke.description.startpage 279 en_US
duke.description.volume 26 en_US
dc.relation.journal AIDS Research and Human Retroviruses en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20218881
pubs.issue 3
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Global Health Institute
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Immunology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Duke Human Vaccine Institute
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery/Surgery, Surgical Sciences Section for AIDS Research & Development
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Human Vaccine Institute
pubs.publication-status Published
pubs.volume 26
dc.identifier.eissn 1931-8405

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