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dc.contributor.author Wang, Jennifer en_US
dc.date.accessioned 2011-04-15T16:46:35Z
dc.date.available 2011-04-15T16:46:35Z
dc.date.issued 2010 en_US
dc.identifier.citation Cortiella,Joaquin;Niles,Jean;Cantu,Andrea;Brettler,Andrea;Pham,Anthony;Vargas,Gracie;Winston,Sean;Wang,Jennifer;Walls,Shannon;Nichols,Joan E.. 2010. Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation. Tissue Engineering Part a 16(8): 2565-2580. en_US
dc.identifier.issn 1937-3341 en_US
dc.identifier.uri http://hdl.handle.net/10161/3318
dc.description.abstract We report here the first attempt to produce and use whole acellular (AC) lung as a matrix to support development of engineered lung tissue from murine embryonic stem cells (mESCs). We compared the influence of AC lung, Gelfoam, Matrigel, and a collagen I hydrogel matrix on the mESC attachment, differentiation, and subsequent formation of complex tissue. We found that AC lung allowed for better retention of cells with more differentiation of mESCs into epithelial and endothelial lineages. In constructs produced on whole AC lung, we saw indications of organization of differentiating ESC into three-dimensional structures reminiscent of complex tissues. We also saw expression of thyroid transcription factor-1, an immature lung epithelial cell marker; prosurfactant protein C, a type II pneumocyte marker; PECAM-1/CD31, an endothelial cell marker; cytokeratin 18; a-actin, a smooth muscle marker; CD140a or platelet-derived growth factor receptor-alpha; and Clara cell protein 10. There was also evidence of site-specific differentiation in the trachea with the formation of sheets of cytokeratin-positive cells and Clara cell protein 10-expressing Clara cells. Our findings support the utility of AC lung as a matrix for engineering lung tissue and highlight the critical role played by matrix or scaffold-associated cues in guiding ESC differentiation toward lung-specific lineages. en_US
dc.language.iso en_US en_US
dc.publisher MARY ANN LIEBERT INC en_US
dc.relation.isversionof doi:10.1089/ten.tea.2009.0730 en_US
dc.subject ii epithelial-cells en_US
dc.subject extracellular-matrix en_US
dc.subject in-vitro en_US
dc.subject 3-dimensional en_US
dc.subject culture en_US
dc.subject pulmonary epithelium en_US
dc.subject engineered lung en_US
dc.subject scaffolds en_US
dc.subject growth en_US
dc.subject constructs en_US
dc.subject geometry en_US
dc.subject cell & tissue engineering en_US
dc.subject biotechnology & applied microbiology en_US
dc.subject cell biology en_US
dc.title Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation en_US
dc.type Article en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-8-0 en_US
duke.description.endpage 2580 en_US
duke.description.issue 8 en_US
duke.description.startpage 2565 en_US
duke.description.volume 16 en_US
dc.relation.journal Tissue Engineering Part a en_US

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