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dc.contributor.author Moutos, FT
dc.contributor.author Guilak, F
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:37Z
dc.date.issued 2010-04
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/19903085
dc.identifier.citation Tissue Eng Part A, 2010, 16 (4), pp. 1291 - 1301
dc.identifier.uri http://hdl.handle.net/10161/3355
dc.description.abstract Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics of native cartilage as the basis for developing functional tissue-engineered constructs. Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured for 28 days in chondrogenic culture conditions. Biomechanical testing showed that PCL-based constructs exhibited baseline compressive and shear properties similar to those of native cartilage and maintained these properties throughout the culture period, while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs displayed an equilibrium coefficient of friction similar to that of native articular cartilage (mu(eq) approximately 0.1-0.3) over the prescribed culture period. Our findings show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced with cartilage-like mechanical properties, and that these engineered properties can be maintained in culture while seeded stem cells regenerate a new, functional tissue construct.
dc.format.extent 1291 - 1301
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof Tissue Eng Part A
dc.relation.isversionof 10.1089/ten.TEA.2009.0480
dc.subject Adult Stem Cells
dc.subject Biocompatible Materials
dc.subject Biomechanical Phenomena
dc.subject Cartilage, Articular
dc.subject Chondrocytes
dc.subject Chondrogenesis
dc.subject Collagen
dc.subject Compressive Strength
dc.subject Extracellular Matrix
dc.subject Fibrin
dc.subject Friction
dc.subject Humans
dc.subject Hydrogels
dc.subject Materials Testing
dc.subject Microscopy, Electron, Scanning
dc.subject Polyesters
dc.subject Regeneration
dc.subject Shear Strength
dc.subject Tensile Strength
dc.subject Tissue Engineering
dc.subject Tissue Scaffolds
dc.title Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-4-0 en_US
duke.description.endpage 1301 en_US
duke.description.issue 4 en_US
duke.description.startpage 1291 en_US
duke.description.volume 16 en_US
dc.relation.journal Tissue Engineering Part a en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/19903085
pubs.issue 4
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.publication-status Published
pubs.volume 16
dc.identifier.eissn 1937-335X

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