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dc.contributor.author Little, D
dc.contributor.author Guilak, F
dc.contributor.author Ruch, DS
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:36Z
dc.date.issued 2010-07
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20406104
dc.identifier.citation Tissue Eng Part A, 2010, 16 (7), pp. 2307 - 2319
dc.identifier.uri http://hdl.handle.net/10161/3364
dc.description.abstract Human adipose stem cells (hASCs) can differentiate into a variety of phenotypes. Native extracellular matrix (e.g., demineralized bone matrix or small intestinal submucosa) can influence the growth and differentiation of stem cells. The hypothesis of this study was that a novel ligament-derived matrix (LDM) would enhance expression of a ligamentous phenotype in hASCs compared to collagen gel alone. LDM prepared using phosphate-buffered saline or 0.1% peracetic acid was mixed with collagen gel (COL) and was evaluated for its ability to induce proliferation, differentiation, and extracellular matrix synthesis in hASCs over 28 days in culture at different seeding densities (0, 0.25 x 10(6), 1 x 10(6), or 2 x 10(6) hASC/mL). Biochemical and gene expression data were analyzed using analysis of variance. Fisher's least significant difference test was used to determine differences between treatments following analysis of variance. hASCs in either LDM or COL demonstrated changes in gene expression consistent with ligament development. hASCs cultured with LDM demonstrated more dsDNA content, sulfated-glycosaminoglycan accumulation, and type I and III collagen synthesis, and released more sulfated-glycosaminoglycan and collagen into the medium compared to hASCs in COL (p <or= 0.05). Increased seeding density increased DNA content incrementally over 28 days in culture for LDM but not COL constructs (p <or= 0.05). These findings suggest that LDM can stimulate a ligament phenotype by hASCs, and may provide a novel scaffold material for ligament engineering applications.
dc.format.extent 2307 - 2319
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof Tissue Eng Part A
dc.relation.isversionof 10.1089/ten.tea.2009.0720
dc.subject Adipose Tissue
dc.subject Adult
dc.subject Animals
dc.subject Collagen
dc.subject DNA
dc.subject Extracellular Matrix
dc.subject Female
dc.subject Gene Expression Regulation
dc.subject Glycosaminoglycans
dc.subject Humans
dc.subject Ligaments
dc.subject Middle Aged
dc.subject Phenotype
dc.subject Stem Cells
dc.subject Sus scrofa
dc.subject Tendons
dc.subject Tissue Scaffolds
dc.title Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells.
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-0 en_US
duke.description.endpage 2319 en_US
duke.description.issue 7 en_US
duke.description.startpage 2307 en_US
duke.description.volume 16 en_US
dc.relation.journal Tissue Engineering Part a en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20406104
pubs.issue 7
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Faculty
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Orthopaedics
pubs.publication-status Published
pubs.volume 16
dc.identifier.eissn 1937-335X

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