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dc.contributor.author Rawson, Jeff en_US
dc.contributor.author Therien, Michael en_US
dc.date.accessioned 2011-06-21T17:22:06Z
dc.date.available 2011-06-21T17:22:06Z
dc.date.issued 2010 en_US
dc.identifier.citation Robbins,Gregory P.;Saunders,Randi L.;Haun,Jered B.;Rawson,Jeff;Therien,Michael J.;Hammer,Daniel A.. 2010. Tunable Leuko-polymersomes That Adhere Specifically to Inflammatory Markers. Langmuir 26(17): 14089-14096. en_US
dc.identifier.issn 0743-7463 en_US
dc.identifier.uri http://hdl.handle.net/10161/3993
dc.description.abstract The polymersome, a fully synthetic cell mimetic, is a tunable platform for drug delivery vehicles to detect and treat disease (theranostics). Here, we design a leuko-polymersome, a polymersome with the adhesive properties of leukocytes, which can effectively bind to inflammatory sites under flow. We hypothesize that optimal leukocyte adhesion can be recreated with ligands that mimic receptors of the two major leukocyte molecular adhesion pathways, the selectins and the integrins. Polymersomes functionalized with sialyl Lewis X and an antibody against ICAM-1 adhere avidly and selectively to surfaces coated with inflammatory adhesion molecules P-selectin and ICAM- I under flow. We find that maximal adhesion occurs at intermediate densities of both sialyl Lewis X and anti-ICAM- I, owing to synergistic binding effects between the two ligands. Leuko-polymersomes bearing these two receptor mimetics adhere under physiological shear rates to inflamed endothelium in an in vitro flow chamber at a rate 7.5 times higher than those to uninflamed endothelium. This work clearly demonstrates that polymersomes bearing only a single ligand bind less avidly and with lower selectivity, thus suggesting proper mimicry of leukocyte adhesion requires contributions from both pathways. This work establishes a basis for the design of polymersomes for targeted drug delivery in inflammation. en_US
dc.language.iso en_US en_US
dc.publisher AMER CHEMICAL SOC en_US
dc.relation.isversionof doi:10.1021/1a1017032 en_US
dc.subject intercellular-adhesion molecule-1 en_US
dc.subject infrared-emissive polymersomes en_US
dc.subject cell-adhesion en_US
dc.subject in-vivo en_US
dc.subject p-selectin en_US
dc.subject endothelial-cells en_US
dc.subject drug-delivery en_US
dc.subject system en_US
dc.subject receptors en_US
dc.subject vesicles en_US
dc.subject chemistry, multidisciplinary en_US
dc.subject chemistry, physical en_US
dc.subject materials science, multidisciplinary en_US
dc.title Tunable Leuko-polymersomes That Adhere Specifically to Inflammatory Markers en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-9-7 en_US
duke.description.endpage 14096 en_US
duke.description.issue 17 en_US
duke.description.startpage 14089 en_US
duke.description.volume 26 en_US
dc.relation.journal Langmuir en_US

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