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dc.contributor.author Saaem, I
dc.contributor.author Ma, KS
dc.contributor.author Marchi, AN
dc.contributor.author LaBean, TH
dc.contributor.author Tian, J
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:22:08Z
dc.date.issued 2010-02
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20356196
dc.identifier.citation ACS Appl Mater Interfaces, 2010, 2 (2), pp. 491 - 497
dc.identifier.issn 1944-8244
dc.identifier.uri http://hdl.handle.net/10161/4000
dc.description.abstract Thermoplastic materials such as cyclic-olefin copolymers (COC) provide a versatile and cost-effective alternative to the traditional glass or silicon substrate for rapid prototyping and industrial scale fabrication of microdevices. To extend the utility of COC as an effective microarray substrate, we developed a new method that enabled for the first time in situ synthesis of DNA oligonucleotide microarrays on the COC substrate. To achieve high-quality DNA synthesis, a SiO(2) thin film array was prepatterned on the inert and hydrophobic COC surface using RF sputtering technique. The subsequent in situ DNA synthesis was confined to the surface of the prepatterned hydrophilic SiO(2) thin film features by precision delivery of the phosphoramidite chemistry using an inkjet DNA synthesizer. The in situ SiO(2)-COC DNA microarray demonstrated superior quality and stability in hybridization assays and thermal cycling reactions. Furthermore, we demonstrate that pools of high-quality mixed-oligos could be cleaved off the SiO(2)-COC microarrays and used directly for construction of DNA origami nanostructures. It is believed that this method will not only enable synthesis of high-quality and low-cost COC DNA microarrays but also provide a basis for further development of integrated microfluidics microarrays for a broad range of bioanalytical and biofabrication applications.
dc.format.extent 491 - 497
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof ACS Appl Mater Interfaces
dc.relation.isversionof 10.1021/am900884b
dc.subject Cycloparaffins
dc.subject DNA
dc.subject Equipment Design
dc.subject Equipment Failure Analysis
dc.subject Membranes, Artificial
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Polymers
dc.title In situ synthesis of DNA microarray on functionalized cyclic olefin copolymer substrate.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-2-0 en_US
duke.description.endpage 497 en_US
duke.description.issue 2 en_US
duke.description.startpage 491 en_US
duke.description.volume 2 en_US
dc.relation.journal Acs Applied Materials & Interfaces en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20356196
pubs.issue 2
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Faculty
pubs.volume 2

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