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dc.contributor.author Ray, Chad en_US
dc.contributor.author Guo, Senli en_US
dc.contributor.author Brown, Dr Jason en_US
dc.contributor.author Li, Nan en_US
dc.contributor.author Akhremitchev, Boris B. en_US
dc.date.accessioned 2011-06-21T17:26:59Z
dc.date.available 2011-06-21T17:26:59Z
dc.date.issued 2010 en_US
dc.identifier.citation Ray,Chad;Guo,Senli;Brown,Jason;Li,Nan;Akhremitchev,Boris B.. 2010. Kinetic Parameters from Detection Probability in Single Molecule Force Spectroscopy. Langmuir 26(14): 11951-11957. en_US
dc.identifier.issn 0743-7463 en_US
dc.identifier.uri http://hdl.handle.net/10161/4086
dc.description.abstract The detection probability of rupture events in A FM force spectroscopy measurements presents a viable alternative to standard methods for extracting kinetic parameters of dissociation. The detection probability has a maximum as a function of the probe velocity where (1) the probability to form a molecular bond is independent of the probe velocity and (2) the detection of rupture events is limited by noise and performed with a constant density of data points per distance of the probe displacement. This newly developed model indicates that the optimal detection velocity is independent of dissociation rate and depends on the distance to the barrier kinetic parameter. Therefore, the kinetic parameters of bond dissociation can be extracted from the dependence of detection probability on probe velocity and the detection threshold. This approach is sensitive to low rupture forces and therefore is complementary to the common most probable force data analysis approach. The developed approach is tested using rupture forces measured with specific bonds between biotin and streptavidin and with nonspecific bonds between linear alkalies in water. Results for the analysis of specific bonds rupture are consistent with the previous measurements, suggesting that rupture forces spanning a wide range of values originate from the same binding potential. Kinetic parameters obtained for linear alkalies are significantly different from previous measurements suggesting possible heterogeneity of the bound state. en_US
dc.language.iso en_US en_US
dc.publisher AMER CHEMICAL SOC en_US
dc.relation.isversionof doi:10.1021/la101269q en_US
dc.subject multiple-bond ruptures en_US
dc.subject ligand-receptor pairs en_US
dc.subject pairwise interactions en_US
dc.subject adhesion bonds en_US
dc.subject microscopy en_US
dc.subject strength en_US
dc.subject water en_US
dc.subject heterogeneity en_US
dc.subject chemistry, multidisciplinary en_US
dc.subject chemistry, physical en_US
dc.subject materials science, multidisciplinary en_US
dc.title Kinetic Parameters from Detection Probability in Single Molecule Force Spectroscopy en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-20 en_US
duke.description.endpage 11957 en_US
duke.description.issue 14 en_US
duke.description.startpage 11951 en_US
duke.description.volume 26 en_US
dc.relation.journal Langmuir en_US

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