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dc.contributor.author Qiu, Ruby en_US
dc.date.accessioned 2011-06-21T17:27:31Z
dc.date.available 2011-06-21T17:27:31Z
dc.date.issued 2010 en_US
dc.identifier.citation Li,Ying;Qiu,Qiong;Watson,Spenser S.;Schweitzer,Ronen;Johnson,Randy L.. 2010. Uncoupling skeletal and connective tissue patterning: conditional deletion in cartilage progenitors reveals cell-autonomous requirements for Lmx1b in dorsal-ventral limb patterning. Development 137(7): 1181-1188. en_US
dc.identifier.issn 0950-1991 en_US
dc.identifier.uri http://hdl.handle.net/10161/4174
dc.description.abstract Integration of muscle, connective tissue and skeletal patterning during development is essential for proper functioning of the musculoskeletal system. How this integration is achieved is poorly understood. There is ample evidence suggesting that skeletal pattern is programmed autonomously, whereas muscle pattern is, for the most part, programmed non-cell-autonomously. Connective tissues depend upon both muscle and skeletal tissues for their proper survival and development. Here, we employed a novel approach to dissect the coordination of musculoskeletal patterning during mouse limb development. Using both conditional gain- and loss-of-function approaches, we selectively deleted or activated the LIM-homeodomain transcription factor Lmx1b in skeletal progenitors using a Sox9-Cre knock-in allele. As Lmx1b is both necessary and sufficient to specify dorsal pattern, this approach allowed us to investigate the effect of selectively deleting or activating Lmx1b in skeletal progenitors on muscle, connective and skeletal tissues during limb development. Our results indicate that whereas Lmx1b activity is required autonomously in skeletal progenitors to direct dorsal pattern, loss or gain of Lmx1b activity in skeletal progenitors has no effect on muscle or connective tissue patterning. Hence, we show for the first time that skeletal and connective tissue patterning can be uncoupled, indicating a degree of autonomy in the formation of the musculoskeletal system. en_US
dc.language.iso en_US en_US
dc.publisher COMPANY OF BIOLOGISTS LTD en_US
dc.relation.isversionof doi:10.1242/dev.045237 en_US
dc.subject limb development en_US
dc.subject dorsal-ventral patterning en_US
dc.subject lmx1b en_US
dc.subject mouse en_US
dc.subject nail-patella syndrome en_US
dc.subject vertebrate limb en_US
dc.subject bud en_US
dc.subject expression en_US
dc.subject muscle en_US
dc.subject musculature en_US
dc.subject origin en_US
dc.subject fate en_US
dc.subject cre en_US
dc.subject developmental biology en_US
dc.title Uncoupling skeletal and connective tissue patterning: conditional deletion in cartilage progenitors reveals cell-autonomous requirements for Lmx1b in dorsal-ventral limb patterning en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-4-1 en_US
duke.description.endpage 1188 en_US
duke.description.issue 7 en_US
duke.description.startpage 1181 en_US
duke.description.volume 137 en_US
dc.relation.journal Development en_US

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