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dc.contributor.author Hutson, MR
dc.contributor.author Zeng, XL
dc.contributor.author Kim, AJ
dc.contributor.author Antoon, E
dc.contributor.author Harward, S
dc.contributor.author Kirby, ML
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:31Z
dc.date.issued 2010-09
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20702561
dc.identifier dev.051565
dc.identifier.citation Development, 2010, 137 (18), pp. 3001 - 3011
dc.identifier.uri http://hdl.handle.net/10161/4176
dc.description.abstract During heart development, a subpopulation of cells in the heart field maintains cardiac potential over several days of development and forms the myocardium and smooth muscle of the arterial pole. Using clonal and explant culture experiments, we show that these cells are a stem cell population that can differentiate into myocardium, smooth muscle and endothelial cells. The multipotent stem cells proliferate or differentiate into different cardiovascular cell fates through activation or inhibition of FGF and BMP signaling pathways. BMP promoted myocardial differentiation but not proliferation. FGF signaling promoted proliferation and induced smooth muscle differentiation, but inhibited myocardial differentiation. Blocking the Ras/Erk intracellular pathway promoted myocardial differentiation, while the PLCgamma and PI3K pathways regulated proliferation. In vivo, inhibition of both pathways resulted in predictable arterial pole defects. These studies suggest that myocardial differentiation of arterial pole progenitors requires BMP signaling combined with downregulation of the FGF/Ras/Erk pathway. The FGF pathway maintains the pool of proliferating stem cells and later promotes smooth muscle differentiation.
dc.format.extent 3001 - 3011
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Development
dc.relation.isversionof 10.1242/dev.051565
dc.subject Animals
dc.subject Arteries
dc.subject Body Patterning
dc.subject Bone Morphogenetic Protein 2
dc.subject Cell Differentiation
dc.subject Cell Lineage
dc.subject Cell Proliferation
dc.subject Chick Embryo
dc.subject Fibroblast Growth Factor 8
dc.subject Gene Expression Regulation, Developmental
dc.subject Heart
dc.subject MAP Kinase Signaling System
dc.subject Muscle, Smooth
dc.subject Myocardium
dc.subject Quail
dc.subject Stem Cells
dc.subject Tissue Culture Techniques
dc.title Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-9-15 en_US
duke.description.endpage 3011 en_US
duke.description.issue 18 en_US
duke.description.startpage 3001 en_US
duke.description.volume 137 en_US
dc.relation.journal Development en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20702561
pubs.issue 18
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pediatrics
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pediatrics/Pediatrics, Neonatology
pubs.publication-status Published
pubs.volume 137
dc.identifier.eissn 1477-9129

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