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dc.contributor.author Rodriguez, P
dc.contributor.author Da Silva, S
dc.contributor.author Oxburgh, L
dc.contributor.author Wang, F
dc.contributor.author Hogan, BL
dc.contributor.author Que, J
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:32Z
dc.date.issued 2010-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/21068065
dc.identifier dev.056077
dc.identifier.citation Development, 2010, 137 (24), pp. 4171 - 4176
dc.identifier.uri http://hdl.handle.net/10161/4178
dc.description.abstract The stratification and differentiation of the epidermis are known to involve the precise control of multiple signaling pathways. By contrast, little is known about the development of the mouse esophagus and forestomach, which are composed of a stratified squamous epithelium. Based on prior work in the skin, we hypothesized that bone morphogenetic protein (BMP) signaling is a central player. To test this hypothesis, we first used a BMP reporter mouse line harboring a BRE-lacZ allele, along with in situ hybridization to localize transcripts for BMP signaling components, including various antagonists. We then exploited a Shh-Cre allele that drives recombination in the embryonic foregut epithelium to generate gain- or loss-of-function models for the Bmpr1a (Alk3) receptor. In gain-of-function (Shh-Cre;Rosa26(CAG-loxpstoploxp-caBmprIa)) embryos, high levels of ectopic BMP signaling stall the transition from simple columnar to multilayered undifferentiated epithelium in the esophagus and forestomach. In loss-of-function experiments, conditional deletion of the BMP receptor in Shh-Cre;Bmpr1a(flox/flox) embryos allows the formation of a multilayered squamous epithelium but this fails to differentiate, as shown by the absence of expression of the suprabasal markers loricrin and involucrin. Together, these findings suggest multiple roles for BMP signaling in the developing esophagus and forestomach.
dc.format.extent 4171 - 4176
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof Development
dc.relation.isversionof 10.1242/dev.056077
dc.subject Animals
dc.subject Bone Morphogenetic Protein Receptors
dc.subject Bone Morphogenetic Protein Receptors, Type I
dc.subject Bone Morphogenetic Proteins
dc.subject Carrier Proteins
dc.subject Epithelium
dc.subject Esophagus
dc.subject Gene Expression Regulation, Developmental
dc.subject Hedgehog Proteins
dc.subject Immunohistochemistry
dc.subject In Situ Hybridization
dc.subject Mice
dc.subject Signal Transduction
dc.subject Stomach
dc.title BMP signaling in the development of the mouse esophagus and forestomach.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-12-15 en_US
duke.description.endpage 4176 en_US
duke.description.issue 24 en_US
duke.description.startpage 4171 en_US
duke.description.volume 137 en_US
dc.relation.journal Development en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/21068065
pubs.issue 24
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Duke Institute for Brain Sciences
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Cell Biology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Neurobiology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pediatrics
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published
pubs.volume 137
dc.identifier.eissn 1477-9129

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