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dc.contributor.author Tvermoes, Brooke E. en_US
dc.date.accessioned 2011-06-21T17:27:35Z
dc.date.available 2011-06-21T17:27:35Z
dc.date.issued 2010 en_US
dc.identifier.citation Tvermoes,Brooke E.;Boyd,Windy A.;Freedman,Jonathan H.. 2010. Molecular characterization of numr-1 and numr-2: genes that increase both resistance to metal-induced stress and lifespan in Caenorhabditis elegans. Journal of cell science 123(12): 2123-2133. en_US
dc.identifier.issn 0021-9533 en_US
dc.identifier.uri http://hdl.handle.net/10161/4187
dc.description.abstract To define the mechanisms involved in the molecular response to the carcinogenic metal cadmium, two novel metal-inducible genes from C. elegans were characterized: numr-1 and numr-2 (nuclear localized metal responsive). numr-1 and numr-2 sequences and cellular patterns of expression are identical, indicating that these are functionally equivalent genes. Constitutive transcription of numr-1 and numr-2 is developmentally regulated and occurs in the intestine, in head and tail neurons, and vulva muscles. Exposure to metals induces numr-1 and numr-2 transcription in pharyngeal and intestinal cells. Other environmental stressors do not affect transcription, indicating that these are metal-specific, stress-responsive genes. NUMR-1 and NUMR-2 target to nuclei and colocalize with HSF-1, suggesting that they may be components of nuclear stress granules. Nematodes overexpressing NUMR-1 and NUMR-2 are resistant to stress and live longer than control animals; likewise reducing expression increases sensitivity to metals and decreases neuromuscular functions. Upstream regulatory regions of both genes contain potential binding sites for DAF-16 and SKN-1, which are components of the insulin-IGF-like signaling pathway. This pathway regulates longevity and stress responses in C. elegans. NUMR-1 and NUMR-2 may function to promote resistance to environmental stressors and longevity, which is mediated by the insulin-IGF-like signaling pathway. en_US
dc.language.iso en_US en_US
dc.publisher COMPANY OF BIOLOGISTS LTD en_US
dc.relation.isversionof doi:10.1242/jcs.065433 en_US
dc.subject c. elegans en_US
dc.subject cadmium en_US
dc.subject stress response en_US
dc.subject lifespan en_US
dc.subject longevity en_US
dc.subject shock transcription factor-1 en_US
dc.subject unfolded protein response en_US
dc.subject cell-specific en_US
dc.subject expression en_US
dc.subject c-elegans en_US
dc.subject cadmium toxicity en_US
dc.subject oxidative stress en_US
dc.subject heavy-metal en_US
dc.subject metallothionein genes en_US
dc.subject regulatory elements en_US
dc.subject signaling pathways en_US
dc.subject cell biology en_US
dc.title Molecular characterization of numr-1 and numr-2: genes that increase both resistance to metal-induced stress and lifespan in Caenorhabditis elegans en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-6-15 en_US
duke.description.endpage 2133 en_US
duke.description.issue 12 en_US
duke.description.startpage 2123 en_US
duke.description.volume 123 en_US
dc.relation.journal Journal of cell science en_US

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