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dc.contributor.author Jones, WM
dc.contributor.author Chao, AT
dc.contributor.author Zavortink, M
dc.contributor.author Saint, R
dc.contributor.author Bejsovec, A
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:36Z
dc.date.issued 2010-07-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20516152
dc.identifier jcs.067868
dc.identifier.citation J Cell Sci, 2010, 123 (Pt 13), pp. 2179 - 2189
dc.identifier.uri http://hdl.handle.net/10161/4189
dc.description.abstract Wg/Wnt signals specify cell fates in both invertebrate and vertebrate embryos and maintain stem-cell populations in many adult tissues. Deregulation of the Wnt pathway can transform cells to a proliferative fate, leading to cancer. We have discovered that two Drosophila proteins that are crucial for cytokinesis have a second, largely independent, role in restricting activity of the Wnt pathway. The fly homolog of RacGAP1, Tumbleweed (Tum)/RacGAP50C, and its binding partner, the kinesin-like protein Pavarotti (Pav), negatively regulate Wnt activity in fly embryos and in cultured mammalian cells. Unlike many known regulators of the Wnt pathway, these molecules do not affect stabilization of Arm/beta-catenin (betacat), the principal effector molecule in Wnt signal transduction. Rather, they appear to act downstream of betacat stabilization to control target-gene transcription. Both Tum and Pav accumulate in the nuclei of interphase cells, a location that is spatially distinct from their cleavage-furrow localization during cytokinesis. We show that this nuclear localization is essential for their role in Wnt regulation. Thus, we have identified two modulators of the Wnt pathway that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
dc.format.extent 2179 - 2189
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof J Cell Sci
dc.relation.isversionof 10.1242/jcs.067868
dc.subject Animals
dc.subject Armadillo Domain Proteins
dc.subject Body Patterning
dc.subject Cell Nucleus
dc.subject Cells, Cultured
dc.subject Cytokinesis
dc.subject Drosophila Proteins
dc.subject Drosophila melanogaster
dc.subject Epistasis, Genetic
dc.subject GTPase-Activating Proteins
dc.subject Genes, Reporter
dc.subject Humans
dc.subject Microtubule-Associated Proteins
dc.subject Phenotype
dc.subject Signal Transduction
dc.subject Transcription Factors
dc.subject Two-Hybrid System Techniques
dc.subject Wings, Animal
dc.subject Wnt Proteins
dc.subject Wnt1 Protein
dc.subject beta Catenin
dc.title Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-1 en_US
duke.description.endpage 2189 en_US
duke.description.issue 13 en_US
duke.description.startpage 2179 en_US
duke.description.volume 123 en_US
dc.relation.journal Journal of cell science en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20516152
pubs.issue Pt 13
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published
pubs.volume 123
dc.identifier.eissn 1477-9137

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