Show simple item record

dc.contributor.author Phelps, JE
dc.contributor.author Vishwanath, K
dc.contributor.author Chang, VT
dc.contributor.author Ramanujam, N
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:27:44Z
dc.date.issued 2010-08-30
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20940771
dc.identifier 205216
dc.identifier.citation Opt Express, 2010, 18 (18), pp. 18779 - 18792
dc.identifier.uri http://hdl.handle.net/10161/4245
dc.description.abstract We developed a ratiometric method capable of estimating total hemoglobin concentration from optically measured diffuse reflectance spectra. The three isosbestic wavelength ratio pairs that best correlated to total hemoglobin concentration independent of saturation and scattering were 545/390, 452/390, and 529/390 nm. These wavelength pairs were selected using forward Monte Carlo simulations which were used to extract hemoglobin concentration from experimental phantom measurements. Linear regression coefficients from the simulated data were directly applied to the phantom data, by calibrating for instrument throughput using a single phantom. Phantoms with variable scattering and hemoglobin saturation were tested with two different instruments, and the average percent errors between the expected and ratiometrically-extracted hemoglobin concentration were as low as 6.3%. A correlation of r = 0.88 between hemoglobin concentration extracted using the 529/390 nm isosbestic ratio and a scalable inverse Monte Carlo model was achieved for in vivo dysplastic cervical measurements (hemoglobin concentrations have been shown to be diagnostic for the detection of cervical pre-cancer by our group). These results indicate that use of such a simple ratiometric method has the potential to be used in clinical applications where tissue hemoglobin concentrations need to be rapidly quantified in vivo.
dc.format.extent 18779 - 18792
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Opt Express
dc.subject Computer Simulation
dc.subject Female
dc.subject Hemoglobins
dc.subject Humans
dc.subject Models, Statistical
dc.subject Monte Carlo Method
dc.subject Optics and Photonics
dc.subject Phantoms, Imaging
dc.subject Precancerous Conditions
dc.subject Regression Analysis
dc.subject Reproducibility of Results
dc.subject Scattering, Radiation
dc.subject Ultraviolet Rays
dc.subject Uterine Cervical Neoplasms
dc.title Rapid ratiometric determination of hemoglobin concentration using UV-VIS diffuse reflectance at isosbestic wavelengths.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-8-30 en_US
duke.description.endpage 18792 en_US
duke.description.issue 18 en_US
duke.description.startpage 18779 en_US
duke.description.volume 18 en_US
dc.relation.journal Optics Express en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20940771
pubs.issue 18
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/Initiatives
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/Initiatives/Duke Science & Society
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Global Health Institute
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published
pubs.volume 18
dc.identifier.eissn 1094-4087

Files in this item

This item appears in the following Collection(s)

Show simple item record