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dc.contributor.author Feng, Sheng en_US
dc.date.accessioned 2011-06-21T17:29:34Z
dc.date.available 2011-06-21T17:29:34Z
dc.date.issued 2010 en_US
dc.identifier.citation Yang,Yunfeng;McCue,Lee Ann;Parsons,Andrea B.;Feng,Sheng;Zhou,Jizhong. 2010. The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control. Bmc Microbiology 10( ): 264-264. en_US
dc.identifier.issn 1471-2180 en_US
dc.identifier.uri http://hdl.handle.net/10161/4352
dc.description.abstract Background: It is well established in E. coli and Vibrio cholerae that strains harboring mutations in the ferric uptake regulator gene (fur) are unable to utilize tricarboxylic acid (TCA) compounds, due to the down-regulation of key TCA cycle enzymes, such as AcnA and SdhABCD. This down-regulation is mediated by a Fur-regulated small regulatory RNA named RyhB. It is unclear in the gamma proteobacterium S. oneidensis whether TCA is also regulated by Fur and RyhB. Results: In the present study, we showed that a fur deletion mutant of S. oneidensis could utilize TCA compounds. Consistently, expression of the TCA cycle genes acnA and sdhA was not down-regulated in the mutant. To explore this observation further, we identified a ryhB gene in Shewanella species and experimentally demonstrated the gene expression. Further experiments suggested that RyhB was up-regulated in fur mutant, but that AcnA and SdhA were not controlled by RyhB. Conclusions: These cumulative results delineate an important difference of the Fur-RyhB regulatory cycle between S. oneidensis and other gamma-proteobacteria. This work represents a step forward for understanding the unique regulation in S. oneidensis. en_US
dc.language.iso en_US en_US
dc.publisher BIOMED CENTRAL LTD en_US
dc.relation.isversionof doi:10.1186/1471-2180-10-264 en_US
dc.subject escherichia-coli en_US
dc.subject vibrio-cholerae en_US
dc.subject superoxide-dismutase en_US
dc.subject target en_US
dc.subject prediction en_US
dc.subject putrefaciens mr-1 en_US
dc.subject iron homeostasis en_US
dc.subject noncoding rnas en_US
dc.subject sequence en_US
dc.subject protein en_US
dc.subject genes en_US
dc.subject microbiology en_US
dc.title The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-10-16 en_US
duke.description.endpage 264 en_US
duke.description.issue en_US
duke.description.startpage 264 en_US
duke.description.volume 10 en_US
dc.relation.journal Bmc Microbiology en_US

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