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dc.contributor.author Granek, JA
dc.contributor.author Magwene, PM
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:16Z
dc.date.issued 2010-01-22
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20107600
dc.identifier.citation PLoS Genet, 2010, 6 (1), pp. e1000823 - ?
dc.identifier.uri http://hdl.handle.net/10161/4461
dc.description.abstract Nutrient stresses trigger a variety of developmental switches in the budding yeast Saccharomyces cerevisiae. One of the least understood of such responses is the development of complex colony morphology, characterized by intricate, organized, and strain-specific patterns of colony growth and architecture. The genetic bases of this phenotype and the key environmental signals involved in its induction have heretofore remained poorly understood. By surveying multiple strain backgrounds and a large number of growth conditions, we show that limitation for fermentable carbon sources coupled with a rich nitrogen source is the primary trigger for the colony morphology response in budding yeast. Using knockout mutants and transposon-mediated mutagenesis, we demonstrate that two key signaling networks regulating this response are the filamentous growth MAP kinase cascade and the Ras-cAMP-PKA pathway. We further show synergistic epistasis between Rim15, a kinase involved in integration of nutrient signals, and other genes in these pathways. Ploidy, mating-type, and genotype-by-environment interactions also appear to play a role in the controlling colony morphology. Our study highlights the high degree of network reuse in this model eukaryote; yeast use the same core signaling pathways in multiple contexts to integrate information about environmental and physiological states and generate diverse developmental outputs.
dc.format.extent e1000823 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS Genet
dc.relation.isversionof 10.1371/journal.pgen.1000823
dc.subject Carbon
dc.subject Cyclic AMP-Dependent Protein Kinases
dc.subject Mutation
dc.subject Nitrogen
dc.subject Saccharomyces cerevisiae
dc.subject Saccharomyces cerevisiae Proteins
dc.title Environmental and genetic determinants of colony morphology in yeast.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-1-0 en_US
duke.description.endpage e1000823 en_US
duke.description.issue 1 en_US
duke.description.startpage e1000823 en_US
duke.description.volume 6 en_US
dc.relation.journal Plos Genetics en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20107600
pubs.issue 1
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Biostatistics & Bioinformatics
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published online
pubs.volume 6
dc.identifier.eissn 1553-7404

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