Show simple item record Chen, SY Wang, Y Telen, MJ Chi, JT
dc.coverage.spatial United States 2011-06-21T17:31:24Z 2008
dc.identifier.citation PLoS One, 2008, 3 (6), pp. e2360 - ?
dc.description.abstract BACKGROUND: Since mature erythrocytes are terminally differentiated cells without nuclei and organelles, it is commonly thought that they do not contain nucleic acids. In this study, we have re-examined this issue by analyzing the transcriptome of a purified population of human mature erythrocytes from individuals with normal hemoglobin (HbAA) and homozygous sickle cell disease (HbSS). METHODS AND FINDINGS: Using a combination of microarray analysis, real-time RT-PCR and Northern blots, we found that mature erythrocytes, while lacking ribosomal and large-sized RNAs, contain abundant and diverse microRNAs. MicroRNA expression of erythrocytes was different from that of reticulocytes and leukocytes, and contributed the majority of the microRNA expression in whole blood. When we used microRNA microarrays to analyze erythrocytes from HbAA and HbSS individuals, we noted a dramatic difference in their microRNA expression pattern. We found that miR-320 played an important role for the down-regulation of its target gene, CD71 during reticulocyte terminal differentiation. Further investigation revealed that poor expression of miR-320 in HbSS cells was associated with their defective downregulation CD71 during terminal differentiation. CONCLUSIONS: In summary, we have discovered significant microRNA expression in human mature erythrocytes, which is dramatically altered in HbSS erythrocytes and their defect in terminal differentiation. Thus, the global analysis of microRNA expression in circulating erythrocytes can provide mechanistic insights into the disease phenotypes of erythrocyte diseases.
dc.format.extent e2360 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0002360
dc.subject Anemia, Sickle Cell
dc.subject Antigens, CD
dc.subject Base Sequence
dc.subject Blotting, Northern
dc.subject DNA Primers
dc.subject Erythrocytes
dc.subject Gene Expression Profiling
dc.subject Genomics
dc.subject Hemoglobin, Sickle
dc.subject Humans
dc.subject MicroRNAs
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Phenotype
dc.subject Receptors, Transferrin
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.title The genomic analysis of erythrocyte microRNA expression in sickle cell diseases.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2008-6-4 en_US
duke.description.endpage e2360 en_US
duke.description.issue 6 en_US
duke.description.startpage e2360 en_US
duke.description.volume 3 en_US
dc.relation.journal Plos One en_US
pubs.issue 6
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Hematology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Rheumatology and Immunology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pathology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Radiation Oncology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published online
pubs.volume 3
dc.identifier.eissn 1932-6203

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