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dc.contributor.author Fuhrman, LE
dc.contributor.author Shianna, KV
dc.contributor.author Aballay, A
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:24Z
dc.date.issued 2008-08-06
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/18682730
dc.identifier.citation PLoS One, 2008, 3 (8), pp. e2882 - ?
dc.identifier.uri http://hdl.handle.net/10161/4499
dc.description.abstract We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.
dc.format.extent e2882 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0002882
dc.subject Animals
dc.subject Caenorhabditis elegans
dc.subject Digestive System
dc.subject Disease Susceptibility
dc.subject Escherichia coli
dc.subject Gene Amplification
dc.subject Genetic Predisposition to Disease
dc.subject Mutagenesis
dc.subject Phenotype
dc.subject Polymorphism, Single Nucleotide
dc.subject Salmonella enterica
dc.title High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2008-8-6 en_US
duke.description.endpage e2882 en_US
duke.description.issue 8 en_US
duke.description.startpage e2882 en_US
duke.description.volume 3 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/18682730
pubs.issue 8
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Duke Institute for Brain Sciences
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.publication-status Published online
pubs.volume 3
dc.identifier.eissn 1932-6203

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