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dc.contributor.author Catron, DJ
dc.contributor.author Noor, MA
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:24Z
dc.date.issued 2008-08-20
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/18714377
dc.identifier.citation PLoS One, 2008, 3 (8), pp. e3009 - ?
dc.identifier.uri http://hdl.handle.net/10161/4502
dc.description.abstract Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes.
dc.format.extent e3009 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0003009
dc.subject Animals
dc.subject Cyclin B
dc.subject Drosophila
dc.subject Drosophila Proteins
dc.subject Gene Expression Profiling
dc.subject Gene Expression Regulation
dc.subject Male
dc.subject Nucleic Acid Hybridization
dc.subject RNA, Messenger
dc.subject Spermatids
dc.subject Spermatogenesis
dc.subject Transcription, Genetic
dc.title Gene expression disruptions of organism versus organ in Drosophila species hybrids.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2008-8-20 en_US
duke.description.endpage e3009 en_US
duke.description.issue 8 en_US
duke.description.startpage e3009 en_US
duke.description.volume 3 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/18714377
pubs.issue 8
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published online
pubs.volume 3
dc.identifier.eissn 1932-6203

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