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dc.contributor.author Mravinac, B
dc.contributor.author Sullivan, LL
dc.contributor.author Reeves, JW
dc.contributor.author Yan, CM
dc.contributor.author Kopf, KS
dc.contributor.author Farr, CJ
dc.contributor.author Schueler, MG
dc.contributor.author Sullivan, BA
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:26Z
dc.date.issued 2009-08-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/19672304
dc.identifier.citation PLoS One, 2009, 4 (8), pp. e6602 - ?
dc.identifier.uri http://hdl.handle.net/10161/4514
dc.description.abstract Human centromeres are multi-megabase regions of highly ordered arrays of alpha satellite DNA that are separated from chromosome arms by unordered alpha satellite monomers and other repetitive elements. Complexities in assembling such large repetitive regions have limited detailed studies of centromeric chromatin organization. However, a genomic map of the human X centromere has provided new opportunities to explore genomic architecture of a complex locus. We used ChIP to examine the distribution of modified histones within centromere regions of multiple X chromosomes. Methylation of H3 at lysine 4 coincided with DXZ1 higher order alpha satellite, the site of CENP-A localization. Heterochromatic histone modifications were distributed across the 400-500 kb pericentromeric regions. The large arrays of alpha satellite and gamma satellite DNA were enriched for both euchromatic and heterochromatic modifications, implying that some pericentromeric repeats have multiple chromatin characteristics. Partial truncation of the X centromere resulted in reduction in the size of the CENP-A/Cenp-A domain and increased heterochromatic modifications in the flanking pericentromere. Although the deletion removed approximately 1/3 of centromeric DNA, the ratio of CENP-A to alpha satellite array size was maintained in the same proportion, suggesting that a limited, but defined linear region of the centromeric DNA is necessary for kinetochore assembly. Our results indicate that the human X centromere contains multiple types of chromatin, is organized similarly to smaller eukaryotic centromeres, and responds to structural changes by expanding or contracting domains.
dc.format.extent e6602 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0006602
dc.subject Animals
dc.subject Base Sequence
dc.subject Centromere
dc.subject Chromosomes, Human, X
dc.subject DNA Methylation
dc.subject DNA Primers
dc.subject Histones
dc.subject Humans
dc.subject Mice
dc.title Histone modifications within the human X centromere region.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2009-8-12 en_US
duke.description.endpage e6602 en_US
duke.description.issue 8 en_US
duke.description.startpage e6602 en_US
duke.description.volume 4 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/19672304
pubs.issue 8
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published online
pubs.volume 4
dc.identifier.eissn 1932-6203

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