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dc.contributor.author Meadows, SK
dc.contributor.author Dressman, HK
dc.contributor.author Daher, P
dc.contributor.author Himburg, H
dc.contributor.author Russell, JL
dc.contributor.author Doan, P
dc.contributor.author Chao, NJ
dc.contributor.author Lucas, J
dc.contributor.author Nevins, JR
dc.contributor.author Chute, JP
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:32Z
dc.date.issued 2010-07-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20634956
dc.identifier.citation PLoS One, 2010, 5 (7), pp. e11535 - ?
dc.identifier.uri http://hdl.handle.net/10161/4550
dc.description.abstract In the event of a terrorist-mediated attack in the United States using radiological or improvised nuclear weapons, it is expected that hundreds of thousands of people could be exposed to life-threatening levels of ionizing radiation. We have recently shown that genome-wide expression analysis of the peripheral blood (PB) can generate gene expression profiles that can predict radiation exposure and distinguish the dose level of exposure following total body irradiation (TBI). However, in the event a radiation-mass casualty scenario, many victims will have heterogeneous exposure due to partial shielding and it is unknown whether PB gene expression profiles would be useful in predicting the status of partially irradiated individuals. Here, we identified gene expression profiles in the PB that were characteristic of anterior hemibody-, posterior hemibody- and single limb-irradiation at 0.5 Gy, 2 Gy and 10 Gy in C57Bl6 mice. These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79-100%) compared to non-irradiated mice. Interestingly, PB signatures of partial body irradiation were poorly predictive of radiation status by site of injury (range 16-43%), suggesting that the PB molecular response to partial body irradiation was anatomic site specific. Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls. These data demonstrate that partial body irradiation, even to a single limb, generates a characteristic PB signature of radiation injury and thus may necessitate the use of multiple signatures, both partial body and total body, to accurately assess the status of an individual exposed to radiation.
dc.format.extent e11535 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011535
dc.subject Animals
dc.subject Female
dc.subject Gene Expression
dc.subject Gene Expression Profiling
dc.subject Leukocytes, Mononuclear
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Radiation Injuries, Experimental
dc.subject Radiation, Ionizing
dc.subject Whole-Body Irradiation
dc.title Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-12 en_US
duke.description.endpage e11535 en_US
duke.description.issue 7 en_US
duke.description.startpage e11535 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20634956
pubs.issue 7
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Faculty
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Global Health Institute
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Social Science Research Institute
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Biostatistics & Bioinformatics
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Immunology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Cellular Therapy
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Hematological Malignancies
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pathology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203

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