Show simple item record Goller, CC Seed, PC
dc.coverage.spatial United States 2011-06-21T17:31:32Z 2010-07-19
dc.identifier.citation PLoS One, 2010, 5 (7), pp. e11642 - ?
dc.description.abstract Rising antibiotic resistance among Escherichia coli, the leading cause of urinary tract infections (UTIs), has placed a new focus on molecular pathogenesis studies, aiming to identify new therapeutic targets. Anti-virulence agents are attractive as chemotherapeutics to attenuate an organism during disease but not necessarily during benign commensalism, thus decreasing the stress on beneficial microbial communities and lessening the emergence of resistance. We and others have demonstrated that the K antigen capsule of E. coli is a preeminent virulence determinant during UTI and more invasive diseases. Components of assembly and export are highly conserved among the major K antigen capsular types associated with UTI-causing E. coli and are distinct from the capsule biogenesis machinery of many commensal E. coli, making these attractive therapeutic targets. We conducted a screen for anti-capsular small molecules and identified an agent designated "C7" that blocks the production of K1 and K5 capsules, unrelated polysaccharide types among the Group 2-3 capsules. Herein lies proof-of-concept that this screen may be implemented with larger chemical libraries to identify second-generation small-molecule inhibitors of capsule biogenesis. These inhibitors will lead to a better understanding of capsule biogenesis and may represent a new class of therapeutics.
dc.format.extent e11642 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011642
dc.subject Anti-Bacterial Agents
dc.subject Antigens, Bacterial
dc.subject Antigens, Surface
dc.subject Bacterial Capsules
dc.subject Escherichia coli
dc.subject Humans
dc.subject Virulence
dc.title High-throughput identification of chemical inhibitors of E. coli Group 2 capsule biogenesis as anti-virulence agents.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-7-19 en_US
duke.description.endpage e11642 en_US
duke.description.issue 7 en_US
duke.description.startpage e11642 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.issue 7
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pediatrics
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pediatrics/Pediatrics, Infectious Diseases
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery/Surgery, Urology
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203

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