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dc.contributor.author Neplioueva, Valentina en_US
dc.contributor.author Dobrikova, Elena en_US
dc.contributor.author Gromeier, Matthias en_US
dc.date.accessioned 2011-06-21T17:31:33Z
dc.date.available 2011-06-21T17:31:33Z
dc.date.issued 2010 en_US
dc.identifier.citation Neplioueva,Valentina;Dobrikova,Elena Y.;Mukherjee,Neelanjan;Keene,Jack D.;Gromeier,Matthias. 2010. Tissue Type-Specific Expression of the dsRNA-Binding Protein 76 and Genome-Wide Elucidation of Its Target mRNAs. Plos One 5(7): e11710-e11710. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10161/4556
dc.description.abstract Background: RNA-binding proteins accompany all steps in the life of mRNAs and provide dynamic gene regulatory functions for rapid adjustment to changing extra-or intracellular conditions. The association of RNA-binding proteins with their targets is regulated through changing subcellular distribution, post-translational modification or association with other proteins. Methodology: We demonstrate that the dsRNA binding protein 76 (DRBP76), synonymous with nuclear factor 90, displays inherently distinct tissue type-specific subcellular distribution in the normal human central nervous system and in malignant brain tumors of glial origin. Altered subcellular localization and isoform distribution in malignant glioma indicate that tumor-specific changes in DRBP76-related gene products and their regulatory functions may contribute to the formation and/or maintenance of these tumors. To identify endogenous mRNA targets of DRBP76, we performed RNA-immunoprecipitation and genome-wide microarray analyses in HEK293 cells, and identified specific classes of transcripts encoding critical functions in cellular metabolism. Significance: Our data suggest that physiologic DRBP76 expression, isoform distribution and subcellular localization are profoundly altered upon malignant transformation. Thus, the functional role of DRBP76 in co- or post-transcriptional gene regulation may contribute to the neoplastic phenotype. en_US
dc.language.iso en_US en_US
dc.publisher PUBLIC LIBRARY SCIENCE en_US
dc.relation.isversionof doi:10.1371/journal.pone.0011710 en_US
dc.subject ribosome entry site en_US
dc.subject nuclear factor-90 en_US
dc.subject ribonucleoprotein complexes en_US
dc.subject mitochondrial biogenesis en_US
dc.subject ilf3 gene en_US
dc.subject nf90 en_US
dc.subject cells en_US
dc.subject identification en_US
dc.subject translation en_US
dc.subject cancer en_US
dc.subject biology en_US
dc.subject multidisciplinary sciences en_US
dc.title Tissue Type-Specific Expression of the dsRNA-Binding Protein 76 and Genome-Wide Elucidation of Its Target mRNAs en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-23 en_US
duke.description.endpage e11710 en_US
duke.description.issue 7 en_US
duke.description.startpage e11710 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US

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