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dc.contributor.author Marguet, P
dc.contributor.author Tanouchi, Y
dc.contributor.author Spitz, E
dc.contributor.author Smith, C
dc.contributor.author You, L
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:33Z
dc.date.issued 2010
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20689598
dc.identifier.citation PLoS One, 2010, 5 (7), pp. e11909 - ?
dc.identifier.uri http://hdl.handle.net/10161/4558
dc.description.abstract Synthetic biology seeks to enable programmed control of cellular behavior though engineered biological systems. These systems typically consist of synthetic circuits that function inside, and interact with, complex host cells possessing pre-existing metabolic and regulatory networks. Nevertheless, while designing systems, a simple well-defined interface between the synthetic gene circuit and the host is frequently assumed. We describe the generation of robust but unexpected oscillations in the densities of bacterium Escherichia coli populations by simple synthetic suicide circuits containing quorum components and a lysis gene. Contrary to design expectations, oscillations required neither the quorum sensing genes (luxR and luxI) nor known regulatory elements in the P(luxI) promoter. Instead, oscillations were likely due to density-dependent plasmid amplification that established a population-level negative feedback. A mathematical model based on this mechanism captures the key characteristics of oscillations, and model predictions regarding perturbations to plasmid amplification were experimentally validated. Our results underscore the importance of plasmid copy number and potential impact of "hidden interactions" on the behavior of engineered gene circuits - a major challenge for standardizing biological parts. As synthetic biology grows as a discipline, increasing value may be derived from tools that enable the assessment of parts in their final context.
dc.format.extent e11909 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011909
dc.subject Escherichia coli
dc.subject Gene Expression Regulation, Bacterial
dc.subject Genes, Synthetic
dc.subject Periodicity
dc.title Oscillations by minimal bacterial suicide circuits reveal hidden facets of host-circuit physiology.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-7-30 en_US
duke.description.endpage e11909 en_US
duke.description.issue 7 en_US
duke.description.startpage e11909 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20689598
pubs.issue 7
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.volume 5
dc.identifier.eissn 1932-6203

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