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dc.contributor.author Zhao, Shengli en_US
dc.contributor.author Gross, Jimmy en_US
dc.contributor.author Miao, Pei en_US
dc.contributor.author Qiu, Li en_US
dc.contributor.author Wang, Dongqing en_US
dc.contributor.author Chen, Qian en_US
dc.contributor.author Feng, Guoping en_US
dc.date.accessioned 2011-06-21T17:32:13Z
dc.date.available 2011-06-21T17:32:13Z
dc.date.issued 2010 en_US
dc.identifier.citation Zhao,Shengli;Zhou,Yang;Gross,Jimmy;Miao,Pei;Qiu,Li;Wang,Dongqing;Chen,Qian;Feng,Guoping. 2010. Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis. Plos One 5(9): e12506-e12506. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10161/4569
dc.description.abstract Neurogenesis in the adult hippocampus is an important form of structural plasticity in the brain. Here we report a line of BAC transgenic mice (GAD67-GFP mice) that selectively and transitorily express GFP in newborn dentate granule cells of the adult hippocampus. These GFP+ cells show a high degree of colocalization with BrdU-labeled nuclei one week after BrdU injection and express the newborn neuron marker doublecortin and PSA-NCAM. Compared to mature dentate granule cells, these newborn neurons show immature morphological features: dendritic beading, fewer dendritic branches and spines. These GFP+ newborn neurons also show immature electrophysiological properties: higher input resistance, more depolarized resting membrane potentials, small and non-typical action potentials. The bright labeling of newborn neurons with GFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and their axon (mossy fiber) terminals, which project to the CA3 region where they form synaptic boutons. GFP expression covers the whole developmental stage of newborn neurons, beginning within the first week of cell division and disappearing as newborn neurons mature, about 4 weeks postmitotic. Thus, the GAD67-GFP transgenic mice provide a useful genetic tool for studying the development and regulation of newborn dentate granule cells. en_US
dc.language.iso en_US en_US
dc.publisher PUBLIC LIBRARY SCIENCE en_US
dc.relation.isversionof doi:10.1371/journal.pone.0012506 en_US
dc.subject neural stem-cells en_US
dc.subject central-nervous-system en_US
dc.subject hippocampal neurogenesis en_US
dc.subject synaptic plasticity en_US
dc.subject glutamic-acid en_US
dc.subject functional implications en_US
dc.subject gabaergic en_US
dc.subject phenotype en_US
dc.subject subventricular zone en_US
dc.subject gyrus en_US
dc.subject neurons en_US
dc.subject biology en_US
dc.subject multidisciplinary sciences en_US
dc.title Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis en_US
dc.title.alternative en_US
dc.description.version Version of Record en_US
duke.date.pubdate 2010-9-2 en_US
duke.description.endpage e12506 en_US
duke.description.issue 9 en_US
duke.description.startpage e12506 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US

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