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dc.contributor.author Ding, L
dc.contributor.author Kuhne, WW
dc.contributor.author Hinton, DE
dc.contributor.author Song, J
dc.contributor.author Dynan, WS
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:32:16Z
dc.date.issued 2010-10-11
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20949019
dc.identifier.citation PLoS One, 2010, 5 (10), pp. e13287 - ?
dc.identifier.uri http://hdl.handle.net/10161/4576
dc.description.abstract BACKGROUND: Small laboratory fish share many anatomical and histological characteristics with other vertebrates, yet can be maintained in large numbers at low cost for lifetime studies. Here we characterize biomarkers associated with normal aging in the Japanese medaka (Oryzias latipes), a species that has been widely used in toxicology studies and has potential utility as a model organism for experimental aging research. PRINCIPAL FINDINGS: The median lifespan of medaka was approximately 22 months under laboratory conditions. We performed quantitative histological analysis of tissues from age-grouped individuals representing young adults (6 months old), mature adults (16 months old), and adults that had survived beyond the median lifespan (24 months). Livers of 24-month old individuals showed extensive morphologic changes, including spongiosis hepatis, steatosis, ballooning degeneration, inflammation, and nuclear pyknosis. There were also phagolysosomes, vacuoles, and residual bodies in parenchymal cells and congestion of sinusoidal vessels. Livers of aged individuals were characterized by increases in lipofuscin deposits and in the number of TUNEL-positive apoptotic cells. Some of these degenerative characteristics were seen, to a lesser extent, in the livers of 16-month old individuals, but not in 6-month old individuals. The basal layer of the dermis showed an age-dependent decline in the number of dividing cells and an increase in senescence-associated β-galactosidase. The hearts of aged individuals were characterized by fibrosis and lipofuscin deposition. There was also a loss of pigmented cells from the retinal epithelium. By contrast, age-associated changes were not apparent in skeletal muscle, the ocular lens, or the brain. SIGNIFICANCE: The results provide a set of markers that can be used to trace the process of normal tissue aging in medaka and to evaluate the effect of environmental stressors.
dc.format.extent e13287 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0013287
dc.subject Aging
dc.subject Animals
dc.subject Biomarkers
dc.subject Eye
dc.subject In Situ Nick-End Labeling
dc.subject Liver
dc.subject Muscle, Skeletal
dc.subject Myocardium
dc.subject Oryzias
dc.subject Skin
dc.title Quantifiable biomarkers of normal aging in the Japanese medaka fish (Oryzias latipes).
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-10-11 en_US
duke.description.endpage e13287 en_US
duke.description.issue 10 en_US
duke.description.startpage e13287 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20949019
pubs.issue 10
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Nicholas School of the Environment
pubs.organisational-group /Duke/Nicholas School of the Environment/Environmental Sciences and Policy
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Civil and Environmental Engineering
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203

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