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dc.contributor.author Gryganskyi, AP
dc.contributor.author Lee, SC
dc.contributor.author Litvintseva, AP
dc.contributor.author Smith, ME
dc.contributor.author Bonito, G
dc.contributor.author Porter, TM
dc.contributor.author Anishchenko, IM
dc.contributor.author Heitman, J
dc.contributor.author Vilgalys, R
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:32:21Z
dc.date.issued 2010-12-09
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/21151560
dc.identifier.citation PLoS One, 2010, 5 (12), pp. e15273 - ?
dc.identifier.uri http://hdl.handle.net/10161/4588
dc.description.abstract The Rhizopus oryzae species complex is a group of zygomycete fungi that are common, cosmopolitan saprotrophs. Some strains are used beneficially for production of Asian fermented foods but they can also act as opportunistic human pathogens. Although R. oryzae reportedly has a heterothallic (+/-) mating system, most strains have not been observed to undergo sexual reproduction and the genetic structure of its mating locus has not been characterized. Here we report on the mating behavior and genetic structure of the mating locus for 54 isolates of the R. oryzae complex. All 54 strains have a mating locus similar in overall organization to Phycomyces blakesleeanus and Mucor circinelloides (Mucoromycotina, Zygomycota). In all of these fungi, the minus (-) allele features the SexM high mobility group (HMG) gene flanked by an RNA helicase gene and a TP transporter gene (TPT). Within the R. oryzae complex, the plus (+) mating allele includes an inserted region that codes for a BTB/POZ domain gene and the SexP HMG gene. Phylogenetic analyses of multiple genes, including the mating loci (HMG, TPT, RNA helicase), ITS1-5.8S-ITS2 rDNA, RPB2, and LDH genes, identified two distinct groups of strains. These correspond to previously described sibling species R. oryzae sensu stricto and R. delemar. Within each species, discordant gene phylogenies among multiple loci suggest an outcrossing population structure. The hypothesis of random-mating is also supported by a 50:50 ratio of plus and minus mating types in both cryptic species. When crossed with tester strains of the opposite mating type, most isolates of R. delemar failed to produce zygospores, while isolates of R. oryzae produced sterile zygospores. In spite of the reluctance of most strains to mate in vitro, the conserved sex locus structure and evidence for outcrossing suggest that a normal sexual cycle occurs in both species.
dc.format.extent e15273 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0015273
dc.subject Alleles
dc.subject Crosses, Genetic
dc.subject DNA Primers
dc.subject DNA, Fungal
dc.subject DNA, Ribosomal
dc.subject Genes, Fungal
dc.subject Genes, Mating Type, Fungal
dc.subject L-Lactate Dehydrogenase
dc.subject Likelihood Functions
dc.subject Models, Genetic
dc.subject Phylogeny
dc.subject Polymerase Chain Reaction
dc.subject Rhizopus
dc.subject Sequence Analysis, DNA
dc.subject Species Specificity
dc.title Structure, function, and phylogeny of the mating locus in the Rhizopus oryzae complex.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-12-9 en_US
duke.description.endpage e15273 en_US
duke.description.issue 11 en_US
duke.description.startpage e15273 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/21151560
pubs.issue 12
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Faculty
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Infectious Diseases
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203

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