Show simple item record

dc.contributor.author Byrnes, EJ
dc.contributor.author Li, W
dc.contributor.author Lewit, Y
dc.contributor.author Ma, H
dc.contributor.author Voelz, K
dc.contributor.author Ren, P
dc.contributor.author Carter, DA
dc.contributor.author Chaturvedi, V
dc.contributor.author Bildfell, RJ
dc.contributor.author May, RC
dc.contributor.author Heitman, J
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:32:22Z
dc.date.issued 2010-04
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20421942
dc.identifier.citation PLoS Pathog, 2010, 6 (4), pp. e1000850 - ?
dc.identifier.uri http://hdl.handle.net/10161/4598
dc.description.abstract Cryptococcus gattii causes life-threatening disease in otherwise healthy hosts and to a lesser extent in immunocompromised hosts. The highest incidence for this disease is on Vancouver Island, Canada, where an outbreak is expanding into neighboring regions including mainland British Columbia and the United States. This outbreak is caused predominantly by C. gattii molecular type VGII, specifically VGIIa/major. In addition, a novel genotype, VGIIc, has emerged in Oregon and is now a major source of illness in the region. Through molecular epidemiology and population analysis of MLST and VNTR markers, we show that the VGIIc group is clonal and hypothesize it arose recently. The VGIIa/IIc outbreak lineages are sexually fertile and studies support ongoing recombination in the global VGII population. This illustrates two hallmarks of emerging outbreaks: high clonality and the emergence of novel genotypes via recombination. In macrophage and murine infections, the novel VGIIc genotype and VGIIa/major isolates from the United States are highly virulent compared to similar non-outbreak VGIIa/major-related isolates. Combined MLST-VNTR analysis distinguishes clonal expansion of the VGIIa/major outbreak genotype from related but distinguishable less-virulent genotypes isolated from other geographic regions. Our evidence documents emerging hypervirulent genotypes in the United States that may expand further and provides insight into the possible molecular and geographic origins of the outbreak.
dc.format.extent e1000850 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS Pathog
dc.relation.isversionof 10.1371/journal.ppat.1000850
dc.subject Animals
dc.subject Cluster Analysis
dc.subject Cryptococcosis
dc.subject Cryptococcus gattii
dc.subject Disease Outbreaks
dc.subject Female
dc.subject Genotype
dc.subject Humans
dc.subject Mice
dc.subject Mitochondria
dc.subject Molecular Epidemiology
dc.subject Northwestern United States
dc.subject Polymerase Chain Reaction
dc.title Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the northwest United States.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-4-0 en_US
duke.description.endpage e1000850 en_US
duke.description.issue 4 en_US
duke.description.startpage e1000850 en_US
duke.description.volume 6 en_US
dc.relation.journal Plos Pathogens en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20421942
pubs.issue 4
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Infectious Diseases
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.volume 6
dc.identifier.eissn 1553-7374

Files in this item

This item appears in the following Collection(s)

Show simple item record