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dc.contributor.author Okagaki, LH
dc.contributor.author Strain, AK
dc.contributor.author Nielsen, JN
dc.contributor.author Charlier, C
dc.contributor.author Baltes, NJ
dc.contributor.author Chrétien, F
dc.contributor.author Heitman, J
dc.contributor.author Dromer, F
dc.contributor.author Nielsen, K
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:32:23Z
dc.date.issued 2010-06-17
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20585559
dc.identifier.citation PLoS Pathog, 2010, 6 (6), pp. e1000953 - ?
dc.identifier.uri http://hdl.handle.net/10161/4602
dc.description.abstract Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological changes in cell size affected pathogenicity via reducing phagocytosis by host mononuclear cells, increasing resistance to oxidative and nitrosative stress, and correlated with reduced penetration of the central nervous system. Cell enlargement was stimulated by coinfection with strains of opposite mating type, and ste3aDelta pheromone receptor mutant strains had reduced cell enlargement. Finally, analysis of DNA content in this novel cell type revealed that these enlarged cells were polyploid, uninucleate, and produced daughter cells in vivo. These results describe a novel mechanism by which C. neoformans evades host phagocytosis to allow survival of a subset of the population at early stages of infection. Thus, morphological changes play unique and specialized roles during infection.
dc.format.extent e1000953 - ?
dc.language ENG
dc.language.iso en_US en_US
dc.relation.ispartof PLoS Pathog
dc.relation.isversionof 10.1371/journal.ppat.1000953
dc.subject Animals
dc.subject Blood-Brain Barrier
dc.subject Blotting, Western
dc.subject Brain
dc.subject Bronchoalveolar Lavage
dc.subject Cell Adhesion
dc.subject Cell Proliferation
dc.subject Cryptococcosis
dc.subject Cryptococcus neoformans
dc.subject Female
dc.subject Flow Cytometry
dc.subject Humans
dc.subject Lung Diseases, Fungal
dc.subject Mice
dc.subject Mice, Inbred A
dc.subject Oxidative Stress
dc.subject Phagocytosis
dc.subject Ploidies
dc.subject RNA, Messenger
dc.subject Receptors, Pheromone
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.title Cryptococcal cell morphology affects host cell interactions and pathogenicity.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US
duke.date.pubdate 2010-6-0 en_US
duke.description.endpage e1000953 en_US
duke.description.issue 6 en_US
duke.description.startpage e1000953 en_US
duke.description.volume 6 en_US
dc.relation.journal Plos Pathogens en_US
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20585559
pubs.issue 6
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Infectious Diseases
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published online
pubs.volume 6
dc.identifier.eissn 1553-7374

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