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dc.contributor.advisor Yuan, Fan en_US
dc.contributor.author Gatti, John WIlliam en_US
dc.date.accessioned 2012-05-25T20:18:36Z
dc.date.available 2012-05-25T20:18:36Z
dc.date.issued 2012 en_US
dc.identifier.uri http://hdl.handle.net/10161/5541
dc.description Thesis en_US
dc.description.abstract <p>A three-dimensional tissue culture device previously described by Elliott et al. 1 is tested for its capacity to mimic cancer interstitial fluid pressure and interstitial pH. The device described by Elliott et al. is a three-channel system; a central channel that contains cells, and two side channels that act as model capillaries. The potential for variation of the interstitial fluid pressure was determined by measuring the resistances with respect to the various channels. It was determined that the unmodified device is incapable of mimicking physiological tumor pressures. The ability to vary pH was performed by using a pH sensitive florescent and by infusing a pH 5.9 solution into the side channels, before checking if the device could maintain this pressure for a long time period during constant perfusion of media into the device. It was observed that the florescence of the pH sensitive molecule decreased upon infusion of an acidic solution, as it should upon exposure to acid. However, no conclusions can be drawn from these tests, as the florescent molecule was no longer viable after the 12 hour perfusion of media. Further experiments are needed before any conclusions can be reached about the device's potential.</p> en_US
dc.subject Biomedical engineering en_US
dc.subject Cancer en_US
dc.subject Cell Culture en_US
dc.subject pH en_US
dc.subject Pressure en_US
dc.title Modification of pH and pressure in a microfluidic cell culture device. en_US
dc.type Thesis en_US
dc.department Biomedical Engineering en_US

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