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dc.contributor.advisor Warren, Warren S en_US
dc.contributor.author Matthews, Thomas en_US
dc.date.accessioned 2012-05-29T16:43:44Z
dc.date.issued 2011 en_US
dc.identifier.uri http://hdl.handle.net/10161/5685
dc.description Dissertation en_US
dc.description.abstract <p>In this dissertation, we develop pump probe spectroscopy as a method to differentiate different chemical varieties of melanin, a common biopigment, and exploit these differences to improve the accuracy of melanoma diagnosis. This method gives insight into the chemical makeup and secondary structure of melanins. Pump probe spectroscopy is implemented in a scanning laser microscope as a form of multiphoton imaging, where it is used to image biopsies of human pigmented cutaneous lesions. Melanoma diagnosis is clinically challenging: the accuracy of visual inspection by dermatologists is highly variable and heavily weighted toward false positives. Even the current gold standard of biopsy results in varying diagnoses among pathologists. Using pump probe imaging, significant chemical and morphological changes were found between melanoma and melanocytic nevi, including increased eumelanin content, chemical heterogeneity and general pigmentation. Signal processing methods revealed further differences between melanoma and melanocytic nevi on the cellular scale. Pump probe imaging directly in H&E stained biopsy samples allows integration of this technique with existing histopathology protocols. High resolution imaging found chemical heterogeneity of melanin within pigmented cells. We show that oxyhemoglobin and deoxyhemoglobin may also be differentiated by pump probe imaging. Epi mode imaging of eumelanin, pheomelanin and microvasculature is demonstrated in vivo in human xenograft mouse models of melanoma.</p> en_US
dc.subject Physical Chemistry en_US
dc.subject Biomedical Engineering en_US
dc.subject Optics en_US
dc.subject melanoma en_US
dc.subject multiphoton imaging en_US
dc.subject pump-probe en_US
dc.title Pump-Probe Molecular Imaging en_US
dc.type Dissertation en_US
dc.department Chemistry en_US
duke.embargo.months 24 en_US
duke.embargo.release 2014-05-19

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