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dc.contributor.advisor York, John D en_US
dc.contributor.author Hatch, Ace Joseph en_US
dc.date.accessioned 2012-09-04T13:14:58Z
dc.date.issued 2012 en_US
dc.identifier.uri http://hdl.handle.net/10161/5777
dc.description Dissertation en_US
dc.description.abstract <p>Inositol phosphates (IPs) are eukaryotic signaling molecules that play important roles in a wide range of biological processes. IPs are required for embryonic development and patterning, insulin secretion, the regulation of telomere length, proper progression through the cell cycle, and the regulation of ion channels. This work uses the yeast Saccharomyces cerevisiae as a model system for investigating the functions of IPs and focuses on the transcriptional regulation of the gene encoding the secreted mating pheromone MF&alpha;2 by the IP kinase Ipk2 (also called Arg82, ArgR3, and IPMK). This work shows that Ipk2 has both kinase-dependent and kinase-independent functions in regulating the transcription of MF&alpha;2. Transcription of MF&alpha;2 is also dependent upon the integrity of an Mcm1-binding site in its promoter. This is the first description of a role for this binding site in the transcription of MF&alpha;2. </p><p><italic>In vivo</italic> and <italic>in vitro</italic> screening approaches to identify additional factors associated with MF&alpha;2 expression or with IP biology generally are also described. These unbiased approaches provide some valuable insight for further investigations.</p> en_US
dc.subject Biochemistry en_US
dc.subject Inositol en_US
dc.subject Phosphate en_US
dc.subject Transcription en_US
dc.subject Yeast en_US
dc.title Investigations of Inositol Phosphate-Mediated Transcription en_US
dc.type Dissertation en_US
dc.department Biochemistry en_US
duke.embargo.months 24 en_US
duke.embargo.release 2014-08-25

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