Wang, ZhengLinden, Lara MNaegeli, Kaleb MZiel, Joshua WChi, QiuyiHagedorn, Elliott JSavage, Natasha SSherwood, David R2014-08-252014-09-01https://hdl.handle.net/10161/9026The receptor deleted in colorectal cancer (DCC) directs dynamic polarizing activities in animals toward its extracellular ligand netrin. How DCC polarizes toward netrin is poorly understood. By performing live-cell imaging of the DCC orthologue UNC-40 during anchor cell invasion in Caenorhabditis elegans, we have found that UNC-40 clusters, recruits F-actin effectors, and generates F-actin in the absence of UNC-6 (netrin). Time-lapse analyses revealed that UNC-40 clusters assemble, disassemble, and reform at periodic intervals in different regions of the cell membrane. This oscillatory behavior indicates that UNC-40 clusters through a mechanism involving interlinked positive (formation) and negative (disassembly) feedback. We show that endogenous UNC-6 and ectopically provided UNC-6 orient and stabilize UNC-40 clustering. Furthermore, the UNC-40-binding protein MADD-2 (a TRIM family protein) promotes ligand-independent clustering and robust UNC-40 polarization toward UNC-6. Together, our data suggest that UNC-6 (netrin) directs polarized responses by stabilizing UNC-40 clustering. We propose that ligand-independent UNC-40 clustering provides a robust and adaptable mechanism to polarize toward netrin.ActinsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell Adhesion MoleculesCell PolarityFemaleIntracellular Signaling Peptides and ProteinsNerve Tissue ProteinsProtein MultimerizationProtein StabilityProtein TransportUterusJournal article1540-8140