Wang, XiaNiu, DechaoLi, PeiWu, QingBo, XiaowanLiu, BojiBao, SongSu, TengXu, HuixiongWang, Qigang2022-12-042022-12-042015-061936-08511936-086Xhttps://hdl.handle.net/10161/26330A dual-enzyme-loaded multifunctional hybrid nanogel probe (SPIO@GCS/acryl/biotin-CAT/SOD-gel, or SGC) has been developed for dual-modality pathological responsive ultrasound (US) imaging and enhanced T2-weighted magnetic resonance (MR) imaging. This probe is composed of functionalized superparamagnetic iron oxide particles, a dual enzyme species (catalase and superoxide dismutase), and a polysaccharide cationic polymer glycol chitosan gel. The dual-modality US/MR imaging capabilities of the hybrid nanogel for responsive US imaging and enhanced T2-weighted MR imaging have been evaluated both in vitro and in vivo. These results show that the hybrid nanogel SGC can exhibit efficient dual-enzyme biocatalysis with pathological species for responsive US imaging. SGC also demonstrates increased accumulation in acidic environments for enhanced T2-weighted MR imaging. Further research on these nanogel systems may lead to the development of more efficient US/MR contrast agents.HumansLiver NeoplasmsPolyethylene GlycolsPolyethyleneimineCatalaseSuperoxide DismutaseMagnetic Resonance ImagingCell SurvivalUltrasonicsBiocatalysisHep G2 CellsMultifunctional EnzymesNanogelsJournal article2022-12-04