Toleman, Clifford ASchumacher, Maria AYu, Seok-HoZeng, WenjieCox, Nathan JSmith, Timothy JSoderblom, Erik JWands, Amberlyn MKohler, Jennifer JBoyce, Michael2020-01-012020-01-012018-060027-84241091-6490https://hdl.handle.net/10161/19689O-GlcNAc is an intracellular posttranslational modification that governs myriad cell biological processes and is dysregulated in human diseases. Despite this broad pathophysiological significance, the biochemical effects of most O-GlcNAcylation events remain uncharacterized. One prevalent hypothesis is that O-GlcNAc moieties may be recognized by "reader" proteins to effect downstream signaling. However, no general O-GlcNAc readers have been identified, leaving a considerable gap in the field. To elucidate O-GlcNAc signaling mechanisms, we devised a biochemical screen for candidate O-GlcNAc reader proteins. We identified several human proteins, including 14-3-3 isoforms, that bind O-GlcNAc directly and selectively. We demonstrate that 14-3-3 proteins bind O-GlcNAc moieties in human cells, and we present the structures of 14-3-3β/α and γ bound to glycopeptides, providing biophysical insights into O-GlcNAc-mediated protein-protein interactions. Because 14-3-3 proteins also bind to phospho-serine and phospho-threonine, they may integrate information from O-GlcNAc and O-phosphate signaling pathways to regulate numerous physiological functions.HumansPhosphopyruvate HydrataseAcetylglucosamine14-3-3 ProteinsProteomicsModels, MolecularMass SpectrometryHEK293 CellsJournal article2020-01-01