Zhou, FeiLi, NingJiang, WeihuaHua, ZhaolaiXia, LinWei, QingyiWang, Liwei2019-02-012019-02-012012-011477-78191477-7819https://hdl.handle.net/10161/17992BACKGROUND: HER-2/neu-targeted therapy has been successfully used in advanced gastric cancer, but the role of HER-2/neu in the prognosis of gastric cancer is not yet clear. In this study, we investigated the correlation between HER-2/neu expression and amplification as well as their association with clinic outcomes in patients with curatively resected gastric cancer. METHODS: We constructed tissue microarray blocks containing >70% of gastric cancer tissue and matched adjacent normal gastric tissue for 227 patients. Expression of the HER-2/neu protein in these specimens was analyzed using immunohistochemical staining. Amplification of HER-2/neu was also analyzed for the same samples using fluorescence in situ hybridization. Data on clinicopathological features and relevant prognostic factors in these patients were analyzed. RESULTS: Of the 227 gastric cancer samples, 11.89% were positive for HER-2/neu overexpression/amplification under the new scoring system. HER-2/neu overexpression/amplification was closely correlated to the Lauren type, degree of differentiation, tumor size and lymph node metastasis. HER-2/neu overexpression/amplification predicted poor survival in univariate analysis but not in a Cox proportional hazards model. CONCLUSION: HER-2/neu overexpression/amplification was not an independent predictor for survival in patients with curatively resected gastric cancer.HumansStomach NeoplasmsNeoplasm InvasivenessLymphatic MetastasisReceptor, erbB-2Neoplasm StagingImmunoenzyme TechniquesPrognosisTissue Array AnalysisIn Situ Hybridization, FluorescenceSurvival RateRetrospective StudiesFollow-Up StudiesGene AmplificationGene Expression Regulation, NeoplasticAdultAgedMiddle AgedChinaFemaleMaleJournal article2019-02-01