Browsing by Author "Cardona, Diana"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Open Access Acute liver failure in the setting of herpes simplex virus and coexistent acute fatty liver of pregnancy(Case Reports in Clinical Pathology, 2014-12-03) Wilder, Julius; Chang, Sydney; Cardona, Diana; Patel, Keyur; Brady, CarlaItem Open Access Assessment of an Online Tool to Simulate the Effect of Pooled Testing for SARS-CoV-2 Detection in Asymptomatic and Symptomatic Populations.(JAMA network open, 2020-12) Polage, Christopher R; Lee, Mark J; Hubbard, Christopher; Rehder, Catherine; Cardona, Diana; Denny, Thomas; Datto, Michael BItem Open Access Early experience with universal preprocedural testing for SARS-CoV-2 in a relatively low-prevalence area.(Infection control and hospital epidemiology, 2020-08-03) Lewis, Sarah S; Smith, Becky A; Akinboyo, Ibukunoluwa C; Seidelman, Jessica; Wolfe, Cameron; Kirk, Allan B; Martin, Gavin; Denny, Thomas; Lobaugh, Bruce; Rehder, Catherine; Cardona, Diana; Lee, Mark J; Polage, Christopher R; Datto, Michael BWe implemented universal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing of patients undergoing surgical procedures as a means to conserve personal protective equipment (PPE). The rate of asymptomatic coronavirus disease 2019 (COVID-19) was <0.5%, which suggests that early local public health interventions were successful. Although our protocol was resource intensive, it prevented exposures to healthcare team members.Item Open Access Histopathologic assessment of cultured human thymus.(PloS one, 2020-01) Hale, Laura P; Neff, Jadee; Cheatham, Lynn; Cardona, Diana; Markert, M Louise; Kurtzberg, JoanneThe maintenance and propagation of complex mixtures of cells in vitro in the form of native organs or engineered organoids has contributed to understanding mechanisms of cell and organ development and function which can be translated into therapeutic benefits. For example, allogeneic cultured postnatal human thymus tissue has been shown to support production of naïve recipient T cells when transplanted into patients with complete DiGeorge anomaly and other genetic defects that result in congenital lack of a thymus. Patients receiving such transplants typically exhibit reversal of their immunodeficiency and normalization of their peripheral blood T cell receptor V-beta repertoire, with long-term survival. This study was designed to assess the histopathologic changes that occur in postnatal human thymus slices when cultured according to protocols used for transplanted tissues. Results showed that as thymic organ cultures progressed from days 0 through 21, slices developed increasing amounts of necrosis, increasing condensation of thymic epithelium, and decreasing numbers of residual T cells. The architecture of the thymic epithelial network remained generally well-preserved throughout the 21 days of culture, with focal expression of cytokeratin 14, a putative biomarker of thymic epithelial cells with long-term organ-repopulating potential. All organ slices derived from the same donor thymus closely resembled one another, with minor differences in size, shape, and relative content of cortex versus medulla. Similarly, slices derived from different donors showed similar histopathologic characteristics when examined at the same culture time point. Taken together, these results demonstrate that diagnostic criteria based on structural features of the tissue identifiable via hematoxylin and eosin staining and cytokeratin immunohistochemistry can be used to evaluate the quality of slices transplanted into patients with congenital athymia.