Browsing by Author "D'Andrea, Alan D"
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Item Open Access Association and prognostic significance of BRCA1/2-mutation status with neoantigen load, number of tumor-infiltrating lymphocytes and expression of PD-1/PD-L1 in high grade serous ovarian cancer(Oncotarget, 2017-07-13) Strickland, Kyle C; Howitt, Brooke E; Shukla, Sachet A; Rodig, Scott; Ritterhouse, Lauren L; Liu, Joyce F; Garber, Judy E; Chowdhury, Dipanjan; Wu, Catherine J; D'Andrea, Alan D; Matulonis, Ursula A; Konstantinopoulos, Panagiotis AItem Open Access Association of Polymerase e–Mutated and Microsatellite-Instable Endometrial Cancers With Neoantigen Load, Number of Tumor-Infiltrating Lymphocytes, and Expression of PD-1 and PD-L1(JAMA Oncology, 2015-12-01) Howitt, Brooke E; Shukla, Sachet A; Sholl, Lynette M; Ritterhouse, Lauren L; Watkins, Jaclyn C; Rodig, Scott; Stover, Elizabeth; Strickland, Kyle C; D'Andrea, Alan D; Wu, Catherine J; Matulonis, Ursula A; Konstantinopoulos, Panagiotis AItem Open Access Clear cell ovarian cancers with microsatellite instability: A unique subset of ovarian cancers with increased tumor-infiltrating lymphocytes and PD-1/PD-L1 expression(OncoImmunology, 2017-02) Howitt, Brooke E; Strickland, Kyle C; Sholl, Lynette M; Rodig, Scott; Ritterhouse, Lauren L; Chowdhury, Dipanjan; D'Andrea, Alan D; Matulonis, Ursula A; Konstantinopoulos, Panagiotis AItem Open Access Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain.(Nucleic Acids Res, 2014-12-16) Wojtaszek, Jessica L; Wang, Su; Kim, Hyungjin; Wu, Qinglin; D'Andrea, Alan D; Zhou, PeiFAAP20 is an integral component of the Fanconi anemia core complex that mediates the repair of DNA interstrand crosslinks. The ubiquitin-binding capacity of the FAAP20 UBZ is required for recruitment of the Fanconi anemia complex to interstrand DNA crosslink sites and for interaction with the translesion synthesis machinery. Although the UBZ-ubiquitin interaction is thought to be exclusively encapsulated within the ββα module of UBZ, we show that the FAAP20-ubiquitin interaction extends beyond such a canonical zinc-finger motif. Instead, ubiquitin binding by FAAP20 is accompanied by transforming a disordered tail C-terminal to the UBZ of FAAP20 into a rigid, extended β-loop that latches onto the complex interface of the FAAP20 UBZ and ubiquitin, with the invariant C-terminal tryptophan emanating toward I44(Ub) for enhanced binding specificity and affinity. Substitution of the C-terminal tryptophan with alanine in FAAP20 not only abolishes FAAP20-ubiquitin binding in vitro, but also causes profound cellular hypersensitivity to DNA interstrand crosslink lesions in vivo, highlighting the indispensable role of the C-terminal tail of FAAP20, beyond the compact zinc finger module, toward ubiquitin recognition and Fanconi anemia complex-mediated DNA interstrand crosslink repair.