Browsing by Author "Dean, J"

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    Massive clonal expansion of medulloblastoma-specific T cells during adoptive cellular therapy.
    (Science advances, 2019-11-27) Flores, C; Wildes, T; Dean, B DiVita; Moore, G; Drake, J; Abraham, R; Gil, J; Yegorov, O; Yang, C; Dean, J; Moneypenny, C; Shin, D; Pham, C; Krauser, J; King, J; Grant, G; Driscoll, T; Kurtzberg, J; McLendon, R; Gururangan, S; Mitchell, D
    In both human and murine systems, we have developed an adoptive cellular therapy platform against medulloblastoma and glioblastoma that uses dendritic cells pulsed with a tumor RNA transcriptome to expand polyclonal tumor-reactive T cells against a plurality of antigens within heterogeneous brain tumors. We demonstrate that peripheral TCR Vβ repertoire analysis after adoptive cellular therapy reveals that effective response to adoptive cellular therapy is concordant with massive in vivo expansion and persistence of tumor-specific T cell clones within the peripheral blood. In preclinical models of medulloblastoma and glioblastoma, and in a patient with relapsed medulloblastoma receiving adoptive cellular therapy, an early and massive expansion of tumor-reactive lymphocytes, coupled with prolonged persistence in the peripheral blood, is observed during effective therapeutic response to immunotherapy treatment.
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    Web Annotation as Conversation and Interruption
    (Media Practice and Education, 2018-01-01) Kalir, JH; Dean, J
    This article showcases both the conventional and disruptive features of web annotation as media practice. To do so, we orchestrated a series of thematic exchanges about media practice, specifically those associated with openness and politics. We then publicly invited responses to our initial manuscript via the online web annotation platform Hypothes.is. The two thematic conversations inspired an ensemble of public contributors to join us in ongoing discussion for over a month, layering atop our source text over 100 original web annotations, creating a laminated and multi-authored document. Following this shared activity, we reflected upon our experience and the generated content, and authored a complementary synthesis that explores the tenor and tensions of web annotation as a disruptive media practice, as well as web annotation as performative publishing. Alongside public contributors, we worked a cyclical dialectic of process and product, discussing web annotation as disruptive media practice by publicly practicing web annotation as an act of co-created disruption. It is our hope that this experiment-turned-article, part collaboratively authored dialogue and part post-hoc synthesis, models and begins to theorize new and disruptive media practices for research design, peer review, and scholarly communication.