Browsing by Author "Tang, Haili"

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    Correction: Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies.
    (PLoS pathogens, 2019-12-02) LaBranche, Celia C; Henderson, Rory; Hsu, Allen; Behrens, Shay; Chen, Xuejun; Zhou, Tongqing; Wiehe, Kevin; Saunders, Kevin O; Alam, S Munir; Bonsignori, Mattia; Borgnia, Mario J; Sattentau, Quentin J; Eaton, Amanda; Greene, Kelli; Gao, Hongmei; Liao, Hua-Xin; Williams, Wilton B; Peacock, James; Tang, Haili; Perez, Lautaro G; Edwards, Robert J; Kepler, Thomas B; Korber, Bette T; Kwong, Peter D; Mascola, John R; Acharya, Priyamvada; Haynes, Barton F; Montefiori, David C
    [This corrects the article DOI: 10.1371/journal.ppat.1008026.].
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    Inhibition of adaptive immune responses leads to a fatal clinical outcome in SIV-infected pigtailed macaques but not vervet African green monkeys.
    (PLoS Pathog, 2009-12) Schmitz, Jörn E; Zahn, Roland C; Brown, Charles R; Rett, Melisa D; Li, Ming; Tang, Haili; Pryputniewicz, Sarah; Byrum, Russell A; Kaur, Amitinder; Montefiori, David C; Allan, Jonathan S; Goldstein, Simoy; Hirsch, Vanessa M
    African green monkeys (AGM) and other natural hosts for simian immunodeficiency virus (SIV) do not develop an AIDS-like disease following SIV infection. To evaluate differences in the role of SIV-specific adaptive immune responses between natural and nonnatural hosts, we used SIV(agmVer90) to infect vervet AGM and pigtailed macaques (PTM). This infection results in robust viral replication in both vervet AGM and pigtailed macaques (PTM) but only induces AIDS in the latter species. We delayed the development of adaptive immune responses through combined administration of anti-CD8 and anti-CD20 lymphocyte-depleting antibodies during primary infection of PTM (n = 4) and AGM (n = 4), and compared these animals to historical controls infected with the same virus. Lymphocyte depletion resulted in a 1-log increase in primary viremia and a 4-log increase in post-acute viremia in PTM. Three of the four PTM had to be euthanized within 6 weeks of inoculation due to massive CMV reactivation and disease. In contrast, all four lymphocyte-depleted AGM remained healthy. The lymphocyte-depleted AGM showed only a trend toward a prolongation in peak viremia but the groups were indistinguishable during chronic infection. These data show that adaptive immune responses are critical for controlling disease progression in pathogenic SIV infection in PTM. However, the maintenance of a disease-free course of SIV infection in AGM likely depends on a number of mechanisms including non-adaptive immune mechanisms.
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    Mapping SARS-CoV-2 antigenic relationships and serological responses.
    (Science (New York, N.Y.), 2023-10) Wilks, Samuel H; Mühlemann, Barbara; Shen, Xiaoying; Türeli, Sina; LeGresley, Eric B; Netzl, Antonia; Caniza, Miguela A; Chacaltana-Huarcaya, Jesus N; Corman, Victor M; Daniell, Xiaoju; Datto, Michael B; Dawood, Fatimah S; Denny, Thomas N; Drosten, Christian; Fouchier, Ron AM; Garcia, Patricia J; Halfmann, Peter J; Jassem, Agatha; Jeworowski, Lara M; Jones, Terry C; Kawaoka, Yoshihiro; Krammer, Florian; McDanal, Charlene; Pajon, Rolando; Simon, Viviana; Stockwell, Melissa S; Tang, Haili; van Bakel, Harm; Veguilla, Vic; Webby, Richard; Montefiori, David C; Smith, Derek J
    During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, multiple variants escaping preexisting immunity emerged, causing reinfections of previously exposed individuals. Here, we used antigenic cartography to analyze patterns of cross-reactivity among 21 variants and 15 groups of human sera obtained after primary infection with 10 different variants or after messenger RNA (mRNA)-1273 or mRNA-1273.351 vaccination. We found antigenic differences among pre-Omicron variants caused by substitutions at spike-protein positions 417, 452, 484, and 501. Quantifying changes in response breadth over time and with additional vaccine doses, our results show the largest increase between 4 weeks and >3 months after a second dose. We found changes in immunodominance of different spike regions, depending on the variant an individual was first exposed to, with implications for variant risk assessment and vaccine-strain selection.
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    Mapping SARS-CoV-2 antigenic relationships and serological responses.
    (bioRxiv, 2022-07-13) Wilks, Samuel H; Mühlemann, Barbara; Shen, Xiaoying; Türeli, Sina; LeGresley, Eric B; Netzl, Antonia; Caniza, Miguela A; Chacaltana-Huarcaya, Jesus N; Corman, Victor M; Daniell, Xiaoju; Datto, Michael B; Dawood, Fatimah S; Denny, Thomas N; Drosten, Christian; Fouchier, Ron AM; Garcia, Patricia J; Halfmann, Peter J; Jassem, Agatha; Jeworowski, Lara M; Jones, Terry C; Kawaoka, Yoshihiro; Krammer, Florian; McDanal, Charlene; Pajon, Rolando; Simon, Viviana; Stockwell, Melissa S; Tang, Haili; van Bakel, Harm; Veguilla, Vic; Webby, Richard; Montefiori, David C; Smith, Derek J
    During the SARS-CoV-2 pandemic, multiple variants with differing amounts of escape from pre-existing immunity have emerged, causing concerns about continued protection. Here, we use antigenic cartography to quantify and visualize the antigenic relationships among 16 SARS-CoV-2 variants titrated against serum samples taken post-vaccination and post-infection with seven different variants. We find major antigenic differences caused by substitutions at spike positions 417, 452, 484, and possibly 501. B.1.1.529 (Omicron BA.1) showed the highest escape from all sera tested. Visualization of serological responses as antibody landscapes shows how reactivity clusters in different regions of antigenic space. We find changes in immunodominance of different spike regions depending on the variant an individual was exposed to, with implications for variant risk assessment and vaccine strain selection. One sentence summary: Antigenic Cartography of SARS-CoV-2 variants reveals amino acid substitutions governing immune escape and immunodominance patterns.